Ifp Energies Nouvelles

Agro Industrie Recherches Et Developpements

L'institut National De La Recherche Agronomique

Institut National De La Recherche Agronomique

Argo Industries Recherche Et Developpement

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Agro Industries Recherche Et Developpement

Fadhel Ben Chaabane

Sylvain Louret

Caroline Aymard

Frederic Monot

Frédéric Monot

Celine Cohen

Etienne Jourdier

Bernard Chaussepied

Céline Cohen

A process for producing oligosaccharides from lignocellulosic biomass, having the following steps:

Process for the production of oligosaccharides from lignocellulosic biomass

The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

Process for the production of cellulolytic and/or hemicellulolytic enzymes

The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising:

Method for producing an enzymatic cocktail from fungal must

The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of COand comprising less than 0.5 molar % of O.

[object Object]

A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases:

Process for the production of an enzymatic cocktail using liquid residues from a process for the biochemical conversion of lignocellulosic materials

The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases:

Process for the production of an enzymatic cocktail using solid residues from a process for the biochemical coversion of lignocellulosic materials

A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h.

Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment

The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.

Growing community of inventors

Paris, France

Fadhel Ben Chaabane