Centre National De La Recherche Scientifique

Centre National De La Recherche (cnrs)

Cnrs and Fabrice Duprat

Fabrice Duprat

Michel Lazdunski

Florian Lesage

Michel Fink

Georges Romey

Eric Honore

Jacques Barhanin

Eric Guillemare

Francois Maingret

Amanda J Patel

A purified protein including a mechanosensitive potassium channel activated by at least one polyunsaturated fatty acid and riluzole and the use of channels in drug screening.

Mechanosensitive mammalian potassium channel activatable by polyunsaturated fatty acids

Human and mouse TREK-1 potassium transport proteins are disclosed as well a method for using potassium transport proteins for identifying substances having anesthetic properties, such as producing a safe, reversible state of unconsciousness with concurrent amnesia and analgesia in a mammal upon inhalation, in a method comprising (a) contacting the test substance with a mammalian potassium transport protein, wherein said potassium transport protein exhibits outward-going potassium rectification; and (b) determining the potassium transport activity of the potassium transport protein, wherein an activation of potassium transport is indicative of the test substance having anesthetic properties.

Method for the identification of anesthetics

This invention relates to the cloning of a member of a new potassium channel named TWIK-1. More specifically, it relates to an isolated and purified nucleic acid molecule coding for a protein constituting a potassium channel exhibiting the proper-ties and structure of the TWIK-1 type channel, as well as the protein coded by this nucleic acid molecule. The invention also relates to the use of this nucleic acid molecule to transform cells, and the use of these cells expressing the potassium channels exhibiting the properties and structure of the TWIK-1 type channel for the screening of drugs.

Isolated TWIK-1 potassium channel proteins

A purified protein comprising a mechanosensitive potassium channel activated by at least one polyunsaturated fatty acid and riluzole and the use of such channels in drug screening.

Method for screening substances capable of modulating the activity of a TRAAK potassium channel

This invention relates to a new member of a recently recognized TWIK potassium,channel family, herein identified as TASK, for TWIK-related acid-sensitive K,channel. This is the first cloned mammalian channel that produces K,currents that possesses all the characteristics of background conductances. The invention also relates to various constructs including the TASK or related human potassium channel family, and their uses.

Family of mammalian potassium channels, their cloning and their use, especially for the screening of drugs

The present invention relates to the cloning of a member of a new potassium channel named TWIK-1. More specifically, it relates to an isolated and purified nucleic acid molecule coding for a protein constituting a potassium channel exhibiting the properties and structure of the TWIK-1 type channel, as well as the protein coded by this nucleic acid molecule. The invention also relates to the use of this nucleic acid molecule to transform cells, and the use of these cells expressing the potassium channels exhibiting the properties and structure of the TWIK-1 type channel for the screening of drugs.

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Vallauris, France

Fabrice Duprat