Massachusetts Institute of Technology

The General Hospital Corporation

Novartis Ag

University of Pennsylvania

Fabio Fachin

Mehmet Toner

Brian Lee Wardle

Ravi Kapur

Kyle C Smith

Thomas Alan Barber

Mathew Varghese

Philipp S Spuhler

Stefan Nikles

Vincent Pai

Michael R Greene

Lan Cao

Nezihi Murat Karabacak

Rodney Rietze

Robert E Cohen

Michael F Rubner

Grace Chen

Murat N Karabacak

Tatiana Golovina

The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

Flow-through paramagnetic particle-based cell separation and paramagnetic particle removal

Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.

Microfluidic sorting using high gradient magnetic fields

Microfluidic sorting with high gradient magnetic fields using opposing arrays of adjacent magnets

The disclosure generally relates to method and apparatus for forming three-dimensional MEMS. More specifically, the disclosure relates to a method of controlling out-of-plane buckling in microstructural devices so as to create micro-structures with out-of-plane dimensions which are 1&#xd7;, 5&#xd7;, 10&#xd7;, 100&#xd7; or 500&#xd7; the film's thickness or above the surface of the wafer. An exemplary device formed according to the disclosed principles, includes a three dimensional accelerometer having microbridges extending both above and below the wafer surface.

Method and apparatus for building three-dimensional MEMS elements

A microfluidic device for manipulating particles can include a substrate and one or more obstacles, each obstacle comprising a plurality of aligned nanostructures including a plurality of nanoparticles or a plurality of polymer layers, or a combination thereof. The obstacle on a substrate can be forests with intra-carbon nanotube spacing ranging between 5-100 nm for isolation of particles such as very small viruses and proteins.

High definition nanomaterials

The devices and systems described herein include one or more fluid paths, e.g., channels, and one or more selectively permeable obstacles arranged in the fluid path(s), each including a plurality of aligned nanostructures, e.g., nanotubes or nanorods, defining an outer surface of the obstacle and an internal network of voids. The obstacle(s) can further include binding moieties applied to the outer surface and/or to the surfaces of the individual nanostructures within the obstacle(s). The devices can be manufactured by forming the dense groupings of nanostructures to extend outwards and upwards from a substrate; forming a fluidic channel, bonding the fluidic channel to the substrate; and optionally applying binding moieties to the obstacles. The devices can be used to manipulate cells within fluid samples.

Microscale and nanoscale structures for manipulating particles

Growing community of inventors

Cambridge, MA, United States of America

Fabio Fachin