Bio-rad Pasteur

Fabienne Babin

Francois Yves Rieunier

François Yves Rieunier

Bernard Pau

Claude Granier

Sylvie Marie-France Trinquier

Catherine Christiane Marie Larue

Charles Didier Calzolari

Odile Suzanne Helene Flecheux

Laurence Hamon

The invention relates to a method of immobilizing an affinity reagent or affinity reagents on a hydrophobic solid phase which can be used in biological assays for analyte detection. The invention relates more particularly to a method of immobilizing an affinity reagent on a hydrophobic solid phase functionalized by a carboxyl group, said method comprising a step for activation of said solid phase and a step for coupling of the affinity reagent to said solid phase, characterized in that the step for activation of said solid phase uses a combination of a carbodiimide and a phosphate buffer in the presence of a co-activator and in an acid medium, and in that the coupling step is performed in a basic medium. The invention further relates to the reactive complexes obtained by this method and to their use in immunoassay kits, hybridization kits or enzymatic assay kits.

Method for immobilization of (an) affinity reagent(s) on a hydrophobic solid phase

The invention relates to synthetic biepitope compounds which can be used as standards in immunoassays, for the assay of troponin I, method for preparing them, compositions and kits containing such compounds as well as the immunoassay methods using such compounds.

Synthetic bioepitope compounds which can be used as standards in the biological assays of Troponin I

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Montigny le Bretonneux, France

Fabienne Babin