Assistance Publique-hopitaux De Paris

UniversitÉ De Cergy Pontoise

Excilone

UniversitÉ Evry Val D'essonne

Cy Cergy Paris Universite

Fabien Piguet

Philippe Manivet

Abdelghani Oukhaled

Hadjer Ouldali

Zuzana Krupova

Juan Pelta

Pierre Defrenaix

An aerolysin nanopore or a nanotube is used for the electrical detection of peptides, proteins separated by at least one amino acid and other macromolecules such as polysaccharides or synthetic or natural polymers present in a preparation where said nanopore or nanotube is inserted into a lipid membrane which is subjected to a difference in potential greater than &#x2212;160 mV, in a reaction medium having an alkali metal halide electrolyte solution with a concentration of less than 6M and at a temperature of less than 40&#xb0; C., and where said use is intended to differentiate said peptides, proteins and other molecules according to their length and their mass. Application to the sequencing of peptides and other molecules to differentiate them according to their length and mass with an amino acid-level or monomer-level resolution and to medical diagnosis.

Electrical detection process for peptides, proteins and other macromolecules

Growing community of inventors

Elancourt, France

Fabien Piguet