Sanofi Pasteur Inc.

Fabien Barbirato

Jérome Le Hir

Nicholas Lindley

Pascal Loubiere

Bo Zhi Sun

Patrick Farrell

Andrew Chiappetta

Javier De Jesus Menendez Diaz

Jerome Le Hir

The present disclosure is directed to a method for cultivating aspecies, comprising: cultivating aspecies under aerobic conditions in a liquid culture medium; and maintaining a pH of the liquid culture medium by using a strong acid, such as nitric acid, or using a first and second acid, wherein the first acid is an inorganic acid that dissociates essentially completely in water, such as nitric acid, hydrochloric acid or sulfuric acid, and wherein the second acid is an inorganic acid having an acid dissociation constant (pKa) of greater than 1, such as phosphoric acid. Methods for increasing the yield offimbrial agglutinogen 2 and fimbrial agglutinogen 3 (FIM2/3) in a supernatant fraction from aculture are also provided.

Methods of cultivatingspecies

The invention concerns a method for producing, on an industrial scale, capsular polysaccharide oftype b (PRP) intended for vaccine purposes, according to which a strain oftype b (Hib) is cultured in a culture medium, the culture supernatant is harvested and treated in order to extract the capsular polysaccharide therefrom, said culture medium comprising at least: &#x2014;one source of carbon, &#x2014;protoporphyrin, &#x2014;salts, &#x2014;amino acids, &#x2014;NAD or NADH, &#x2014;vitamins, &#x2014;means for regulating the pH, characterized in that said culture medium is chemically defined.

[object Object]

The invention concerns a method for producing, on an industrial scale, capsular polysaccharide oftype b (PRP) intended for vaccine purposes, according to which a strain oftype b (Hib) is cultured in a culture medium, the culture supernatant is harvested and treated in order to extract the capsular polysaccharide therefrom, said culture medium comprising at least: &#x2014;one source of carbon, &#x2014;protoporphyrin, &#x2014;salts, &#x2014;amino acids, &#x2014;NAD or NADH, &#x2014;vitamins, &#x2014;means for regulating the pH, characterised in that said culture medium is chemically defined.

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Brignais, France

Fabien Barbirato