Mesoscale Technologies, Llc.

Igen International, Inc.

Igen, Inc.

Meso Scale Technology Llp

Fabian Jameison

Jonathan K Leland

Mark T Martin

Alan B Fischer

Robert Hoch

Richard J Massey

Liwen Dong

Pam Liang

Ming Lu

Graphitic nanotubes, which include tubular fullerenes (commonly called 'buckytubes') and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

Graphitic nanotubes in luminescence assays

Graphitic nanotubes, which include tubular fullerenes (commonly called &ldquo;buckytubes&rdquo;) and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

An electrochemiluminescence (ECL)-based assay for the quantitation of classical clinical chemistry analytes, where glucose, ethanol, carbon dioxide, cholesterol, as H.sub.2 O.sub.2 liberating systems coupled to a tris(bipyridyl)ruthenium(II) Ru(bpy).sub.3.sup.2+ luminophore generate an ECL signal, which can be detected spectrophotometrically or electrochemically. The H.sub.2 O.sub.2 present reacts with oxalate in the presence of Ru(bpy).sub.3.sup.2+ to give an ECL response.

Hydrogen peroxide based ECL

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Gaithersburg, MD, United States of America

Fabian Jameison