Pacific Biosciences of California, Inc.

Omniome, Inc.

Fabian Block

Brittany Ann Rohrman

Arnold Oliphant

Pinar Iyidogan

Morassa Mohseni Middleton

Mark C Wallen

Ying Lin Liu

Michael James Schmidt

Hari K K Subramanian

Kurt Patterson

A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.

Methods and compositions for sequencing double stranded nucleic acids

Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca. In addition to Li, the fluid can contain polyethylenimine (PEI) with or without betaine.

Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes

Methods and compositions for sequencing double stranded nucleic acids using RCA and MDA

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San Diego, CA, United States of America

Fabian Block