Somalogic, Incorporated

Evaldas Katilius

Nebojsa Janjic

Urs Ochsner

Larry M Gold

Dominic Zichi

Stephan Kraemer

Glenn Sanders

Edgar Olivas

The present disclosure relates generally to the field of nucleic acids and, more particularly, to aptamers capable of binding to toxins produced by; diagnostic kits and methods comprising such aptamers; and methods of making and using such aptamers.

Aptamers for clostridium difficile diagnostics

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The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.

Method for purification and identification of sperm cells

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Superior, CO, United States of America

Evaldas Katilius