Miles Inc.

Bayer Corporation

Eva Sajan

Willie J Lewis

Raleigh A Carmen

Edward J Nelson

Ronald H Bauman

Charles V Olson

Michael Zachariou

Ricaredo Matanguihan

Donald R Denton

William W Dupin

A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.

Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration

Plastic blood bag system including a bag having a generally longitudinal seal extending from the top of the bag and between two blood bag ports downwardly to at least the lower half of the bag but not to the bottom of the bag, thereby leaving the sole passageway within the bag and between two ports located in the lower half of the bag. The longitudinal seal terminates proximal to the central axis of the bag with a spot weld to prevent tear. In addition, the cross-sectional diameter of the channel increases as the channel nears the central axis.

Bottom blood bag separation system

Plastic blood bag system including a bag having a generally longitudinal seal extending from the top of the bag and between two blood bag ports downwardly to at least the lower half of the bag but not to the bottom of the bag, thereby leaving the sole passageway within the bag and between two ports located in the lower half of the bag.

Blood bag system for separating whole blood into upper, middle, and lower components which can then be isolated from each other in an automated manner. The system comprises a primary or main blood bag having first and second outlet ports. The first outlet port communicates via tubing with a first satellite bag. The second outlet port communicates via tubing with a second satellite bag at one end and at the other end communicates with a tubular member extending into and toward the bottom of the main bag. In use whole blood is drawn into the main bag through an inlet port and centrifuged to form an upper, less dense plasma portion, a lower, more dense red blood cell (RBC) portion and an intermediate portion of buffy coat (including platelets). Pressure is then applied to the bag with the first outlet open and the second outlet closed to express all of the upper plasma through the first outlet port after which the first outlet port is closed and the second outlet is opened. The lower RBCs are expressed by pressure through the tubular member and second outlet, leaving only the intermediate buffy coat in the main bag. The system may be automated with sensors to monitor and control the flow of fluids through each outlet and from the main bag.

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Oakland, CA, United States of America

Eva Sajan