Ge Healthcare Bio-sciences Ab

Cytiva Bioprocess R&d Ab

Ge Healthcare Bioprocess R&d Ab

Eva Holmgren

Anders Larsson

Hans Berg

Henrik Ihre

Dag Lindstrom

Anders Hagvall

Maria Brännholm

David Buckley

Maria Birgitta Holm

Dag Lindstroem

Ronnie Palmgren

Philippe Busson

Per Erik Emilsson

Jimmy Hedin Dahlstrom

Jonas Bengtsson

Maria Holm

Maranda Fordham

Fernando Polanco

Dag Lindström

A system and method for producing fine droplets of polysaccharide from a premixed water-in-oil emulsion uses a packed bed () comprising hydrophilic beads ().

Packed bed emulsification

The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.

Method of preparing a separation matrix

The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.

Method of preparing a chromatography matrix

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Uppsala, Sweden

Eva Holmgren