Biogal Gyogyszergyar Rt.

Teva Gyogyszergyar Zartkoruen Mukodo Reszvenytarsasag

Eva Gulyas

Janos Erdei

Gabor Balogh

Istvan M Szabo

Istvan Barta

Karoly Albrecht

Valeria Szell

Julianna Mozes Nee Suto

Ildiko Lang

Aniko Tedges

Magdolna Petroczki

Vilmos Keri

Andrea Csorvasi

Peter Seress

Laszlo Toth

An improved fermentation process for preparing pseudomonic acid A (mupirocin) is disclosed. The metabolically controlled fermentation process provides culturing asp. strain in a submerged medium at a temperature within about 20-30&#xb0; C. The pH of the fermentation medium is regulated to be at about 5.5-6.0 by feeding the fermentation medium with an assimilable carbon source, a mineral salt, or an acidic/alkali solution. Accordingly, the resulting fermentation broth contains an increased yield of highly purified pseudomonic acid A as the main component. The pseudomonic acid B as an impurity in the fermentation broth is significantly decreased.

pH controlled fermentation process for pseudomonic acid production

A procedure for the preparation of pseudomonic acid A comprising submerged cultivation of abacterium strain capable of biosynthesis of the substantially pure pseudomonic acid A in aerated conditions via fermentation; and isolation of the desired compound is disclosed. In particular, the procedure of the present invention comprises cultivation of thesp. bacterium strain No. 19/26 deposited under accession No. NCAIM(P)B 001235 in the National Collection of the Agricultural and Industrial Microorganisms, Budapest, Hungary, or its pseudomonic acid A-producing mutant or variant, on a medium at a temperature of between about 20&#xb0; C. and 30&#xb0; C. containing organic nitrogen and carbon sources and, optionally, mineral salts.

Process for the preparation of pseudomonic acid a antibiotic by microbiological method

The present invention provides a fermentation process for producing lipstatin comprising the steps of: a) preparing a fermentation medium containing a lipstatin-producing microorganism comprising an oil and an assimilable carbon source, wherein the wt/wt ratio of oil and assimilable carbon source is regulated to achieve an optimal lipstatin biosynthesis by the microorganism; and b) feeding the fermentation medium with an emulsifier, wherein the emulsifier provides an optimal viscosity for the fermentation medium and optimal pH during the fermentation to permit fermentation for lipstatin production. The disclosed process also provides a process for extracting a lipstatin from a fermentation broth.

Fermentation process for lipstatin and method of extracting lipstatin from a fermentation broth

A procedure for the preparation of pseudomonic acid A comprising submerged cultivation of a Pseudomonas bacterium strain capable of biosynthesis of the substantially pure pseudomonic acid A in aerated conditions via fermentation; and isolation of the desired compound is disclosed. In particular, the procedure of the present invention comprises cultivation of the Pseudomonas sp. bacterium strain No. 19/26 deposited under accession No. NCAIM(P)B 001235 in the National Collection of the Agricultural and Industrial Microorganisms, Budapest, Hungary, or its pseudomonic acid A-producing mutant or variant, on a medium at a temperature of between about 20&deg; C. and 30&deg; C. containing organic nitrogen and carbon sources and, optionally, mineral salts.

Process for the preparation of pseudomonic acid A antibiotic by microbiological method

Growing community of inventors

Debrecen, Hungary

Eva Gulyas