Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Eva Cecilia Olsson

Johan Arthursson

Lena Nyholm

Lotta Hedkvist

Andreas Andersson

Christian Elnegard

Fredrik Torgny Nilsson

Ulrika Ohman

Henrik Larsson

Subasish Mohapatra

Jens Ornberg

Erik Gunnarsson

Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: &#x2014;performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and&#x2014;displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.

Method and system for determining the influence of experimental parameters on a liquid chromatography protocol

The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter, identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s) in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.

Method and apparatus for performing liquid chromatography purification

Display screen with graphical user interface

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Uppsala, Sweden

Eva Cecilia Olsson