Akzo Nobel N.v.

Eun Mi Lee

Joseph W Romano

Gregory J Hurteau

Yuexia Li

Kathy Lynn Hickman

An isothermal transcription based amplification assay for dengue virus RNA uses primer combinations for sequences within the envelope gene or the 3&prime; non-coding region of the virus and a probe. Probes may be specific for a serotype of dengue virus.

Isothermal transcription based assay for the detection and genotyping of dengue virus

A method and a test kit for genotyping the CCR5 chemokine receptor. CCR5 transcript DNA is amplified using transcription based amplification. The amplification product is detected through the hybridization of appropriately labeled oligonucleotide wild type and mutant probes. In a liquid hybridization based ECL analysis, the mutant probe is a 16mer having the sequence 5&prime;TCCATACATTAAAGAT3&prime; &lsqb;SEQ ID NO:9&rsqb;. The method and kit can be used to predict.

CCR5 RNA transcription based amplification assay

The present invention provides in situ amplification methods for the detection of a target nucleic acid molecule. The methods use a molecule termed &ldquo;molecular tape&rdquo; to retain the amplified target nucleic acid molecule within a cell upon amplification. The molecular tape, which may be used with a variety of amplification methods, improves the in situ detection of an amplified target molecule.

Methods and reagents for in situ amplification

An isothermal transcription based amplification assay for dengue virus RNA uses primer combinations for sequences within the envelope gene or the 3' non-coding region of the virus and a probe. Probes may be specific for a serotype of dengue virus.

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Gaithersburg, MD, United States of America

Eun Mi Lee