Korea Research Institute of Bioscience and Biotechnology

Eui Sung Choi

Jung Oh Ahn

Eun Gyo Lee

Hong Weon Lee

Joon Ki Jung

Hyeok Won Lee

Chun Suk Kim

Myoung Soo Park

Ji Yeon Hong

Seon Won Kim

Jae Ik Whang

Hee Kyoung Ryu

The present invention relates to a modified yeast strain which is prepared by introducing a vector that expresses HMG-CoA reductase (hydroxymethylglutaryl CoA reductase) and farnesyl pyrophosphate synthase, and a method for producing squalene using the same. More particularly, the present invention relates toY2805 modified yeast strain that is transformed with a vector including the HMG1 gene, and ispA or Erg20 gene, and a method for producing squalene with high efficiency by culturing the modified yeast strain.

Modified yeast strain and a method for producing squalene using the same

Disclosed herein are a-derived auto-inducible NPS promoter; a recombinant expression vector carrying the promoter and a nucleotide sequence, the sequence being operably linked to the promoter and coding for a recombinant protein; a host cell transformed or transfected with the recombinant expression vector; and a method for producing a recombinant protein, comprising culturing the host cell to express the recombinant protein and isolating the protein. The NPS promoter allows a recombinant protein of interest to be produced on a large scale without the need of any inducer.

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Disclosed are a-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express the-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.

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Daejeon, South Korea

Eui Sung Choi