Nanogen, Inc.

Biological Dynamics, Inc.

Omniome, Inc.

Nanomix, Inc.

Gamida for Life B.v.

Genoptix, Inc.

Celula, Inc.

Pacific Biosciences of California, Inc.

Veriskin, Inc.

Dynamic Connections, LLC

Nanotrope Inc.

Eugene Tu

Michael James Heller

David Y Liu

Paul David Swanson

James McCanna

Robert Paul Turner

Rajaram Krishnan

Kai Yang

David Jean Charlot

Irina V Dobrovolskaya

Lucas Kumosa

William Frank Butler

Ronald George Sosnowski

Kandaswamy Vijayan

Carl Frederick Edman

Mark Wang

Corey M Dambacher

Mirianas Chachisvilis

James P O'Connell

Mark A Bernard

Joseph Franklin Rokicki

Christian Valcke

Kristie L Lykstad

Kerry Wilson

Michael I Nerenberg

Juan Pablo Hinestrosa Salazar

Haichuan Zhang

Philippe Marchand

Glen A Evans

Juscilene Menezes

Norbert D Hagen

Yi Zhang

Rong Yang

Ying-Lan Chang

Jean-Christophe P Gabriel

Keith Bradley

John Loren Passmore

Thomas H Marsilje, Iii

Ray R Radtkey

Sergei Skarupo

Osman Kibar

Christian Gurtner

Lorelei P Westin

Daniel E Raymond

Mikhail Briman

Robert Dees

Joon Mo Yang

Donald E Ackley

Steven T Brentano

Keunho Ahn

Michael Nghiem Nguyen

William F Butler

Devon Cayer

Michael Krihak

Andrew S Katz

Anita Forster

Kurt A Krummel

John Francis

Benjamin Lai

Richard LeCoultre

Russell Baldocchi

Richard Lecoultre

Mark M Wang

David Liu

Kerry Gunning

Phillippe Marchand

Irina Dobrovolskaya

Rama Ghatti

A method and a device for diagnostic of skin cancer and other mammalian skin tissue pathologies are described. The method relies on determination of pathological changes in tissue vascularization and capillary blood flow. The device uses photonic emitters and detectors to characterize temporal and spatial changes in blood flow in response to external perturbation such as external mechanical force or temperature change.

Skin perfusion monitoring device

The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides. The methods rely, in part, on identifying a base of a template nucleic acid during nucleic acid synthesis by controlling the sequencing reaction conditions. Template nucleic acid bases may be identified during an examination step followed by an optional incorporation step.

Nucleic acid sequencing methods and systems

The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.

Methods of quantifying target nucleic acids and identifying sequence variants

Method of identifying a cognate nucleotide (i.e., the 'next correct nucleotide') for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.

Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides

A method for separating a target allele from a mixture of nucleic acids by (a) providing a mixture of nucleic acids in fluidic contact with a stabilized ternary complex that is attached to a solid support, wherein the stabilized ternary complex includes a polymerase, primed nucleic acid template, and next correct nucleotide, wherein the template has a target allele, wherein the next correct nucleotide is a cognate nucleotide for the target allele, and wherein the stabilized ternary complex is attached to the solid support via a linkage between the polymerase and the solid support or via a linkage between the next correct nucleotide and the solid support; and (b) separating the solid support from the mixture of nucleic acids, thereby separating the target allele from the mixture of nucleic acids.

Allele-specific capture of nucleic acids

Provided are compositions, methods and systems for determining the sequence of a template nucleic acid using a polymerase-based, sequencing-by-binding procedure. An examination step involves monitoring the interaction between a polymerase and template nucleic acid in the presence of one or more nucleotides. Identity of the next correct nucleotide in the sequence is determined without incorporation of any nucleotide into the structure of the primer by formation of a phosphodiester bond. An optional incorporation step can be used after the examination step to extend the primer by one or more nucleotides, thereby incrementing the template nucleotides that can be examined in a subsequent examination step. The sequencing-by-binding procedure does not require the use of labeled nucleotides or polymerases, but optionally can employ these reagents.

Method and system for sequencing nucleic acids

Provided herein are methods for analyzing a signature sequence in a nucleic acid sample by rapid sequencing of a target nucleic acid region. The method examines the target nucleic acid directly and minimizes the number of examination steps needed to determine a signature that is characteristic of a genetic feature of the nucleic acid sample.

Methods for determining sequence profiles

The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.

Nucleic acid sample preparation

Growing community of inventors

San Diego, CA, United States of America

Eugene Tu