Petroleum Fermentations N.v.

Other

Biotechnologie Aktiengesellschaft Fur Emulsan

Ramot University Authority for Applied Research and Industrial

Biological Petroleum Cleaning Ltd.

Tel Aviv University

Mekorot Water Company Ltd.

Petroleum Fermentations

Eugene Rosenberg

David L Gutnick

Igal Belsky

Zosim Zinaida

Eliora Z Ron

Eliora Ron

David Amir

Gili Bittan-Banin

Yuval Shoham

Eirik Nestaas

Ram Cohen

Elisha Orr

Yossi Paitan

David Gutnick

Yossef Shabtai

Zinaida Zosim

Nechemia Sar

Provided is a method of bioremediation of organic contaminated water within an annulus of an oil lubricated shaft, the method includes: (a) introducing into the annulus of said oil lubricated shaft an amount of a bioremediation combination including (i) a microbial cocktail capable of biodegrading the organic contaminants and (ii) nitrogen source, such that said bioremediation combination is brought into contact with water suspected of being contaminated in said annulus; and (b) supply into said annulus oxygen source for a time sufficient for biodegradation of at least a portion of said organic contaminants in the water within the annulus.

Method for bioremediation of contaminated water

A system and method for treating wastewater by continuously flowing wastewater into a chemostat and continuously discharging clean water out of the chemostat. The system can include sensors and an electronic controller for on-line measuring ambient parameters in the chemostat and adjusting the chemostat's operating conditions accordingly.

Wastewater treatment

There is provided DNA sequences isolated frompartially encoding a functional portion of a polypeptide component required for the synthesis of antibiotic TA. Also provided are purified, isolated and cloned DNA sequences encoding a polypeptide component required for postmodification of antibiotic TA and encoding a gene product involved in the regulation of the biosynthesis of antibiotic TA. A purified, isolated and cloned DNA sequence having a DNA sequence (seq. ID No:2 and 20) encoding a polypeptide component required for encoding the TA gene cluster and any mutations thereof is provided. Also provided are methods of using the TA genes for combinatorial genetics and of using the TA genes encoding for synthesis and modification or regulation of antibiotic TA.

DNA encoding a polypeptide required for biosynthesis of TA antibiotic

Bioemulsifiers

The present invention relates to compositions containing bacteria capable of degrading hydrocarbons, such as petroleum or petroleum products and methods for their use.

Non-polluting compositions to degrade hydrocarbons and microorganisms

The present invention relates to polymeric dispersing agents for finely divided materials, particularly minerals, which are fermentation products of bacteria. The invention relates to bacterial strains producing such dispersants and to a process for the production of the dispersants by fermentation of such bacteria. The invention further relates to compositions containing effective quantities of such polymeric fermentation products in order to be effective dispersants for minerals.

Bacterial process for the production of dispersants

Methods and compositions are provided for the preparation and use of new nondialyzable, interfacially-active bioemulsifiers from Acinetobacter calcoaceticus strains.

Bioemulsifier production by Acinetobacter calcoaceticus strains

Methods and compositions are provided for the production and use of enzymes that degrade lipopolysaccharide bioemulsifiers, and, in particular, emulsans. The enzymes may be used to demulsify bioemulsifier-stabilized hydrocarbon-in-water emulsions.

Enzymatic degradation of lipopolysaccharide bioemulsifiers

Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the 'emulsans') which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deacylated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

.alpha.Emulsans

Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the 'emulsans') which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deacylated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

Polyanionic heteropolysaccharide biopolymers

Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the 'emulsans') which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the '.psi.-emulsans' and 'apo-.psi.-emulsans', respectively) which are completely N-acylated and partially to completely O-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbiol polysaccharides as well as their O-deacylated and N-deacylated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

.psi.-Emulsans

Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the 'emulsans') which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Deproteinization of emulsans by hot phenol extraction produces the lipopolysaccharide components (the 'apoemulsans') of each of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of a major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apoemulsans containing varying percentages of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the '.psi.-emulsans' and 'apo-.psi.-emulsans', respectively) which are completely N-acylated and partially to completely O-deacylated. Base hydrolysis under strong conditions of any of these products produces another derivate (the 'proemulsans') which is completely O-deacylated and is partially N-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deaclated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

Apo-.alpha.-emulsans

Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the 'emulsans') which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Deproteinization of emulsans by hot phenol extraction produces the lipopolysaccharide components (the 'apoemulsans') of each of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of a major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apoemulsans containing varying percentages of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the '.psi.-emulsans' and 'apo-.psi.-emulsans', respectively) which are completely N-acylated and partially to completely O-deacylated. Base hydrolysis under strong conditions of any of these products produces another derivate (the 'proemulsans') which is completely O-deacylated and is partially N-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deaclated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

Growing community of inventors

Raanana, Israel

Eugene Rosenberg