Other

University of California

Prototek, Inc.

Enzyme Systems Products

Eugene R Bissell

Robert E Smith

Alexander R Mitchell

Mary P Zimmerman

David W Rasnick

Robert F Smith

Karen W Pearson

Amino acid substituted-cresyl violet, synthetic fluorogenic substrates for the analysis of agents in individual in vivo cells or tissue

A method of treating medical conditions with proteinase inhibitors of the formula: ##STR1## wherein B is H or an amino acid blocking group for the N-terminal amino acid nitrogen;

Cysteine protease inhibitors effective for in vivo use

Substrates and their corresponding pharmaceutically acceptable acid salts derived from 7-amino-4-trifluoromethyl-quinolone are disclosed. These substrates are useful for determining the presence of an enzyme or enzyme inhibitor in a biological fluid, wherein the fluid is contacted with a substrate. This mixture is thereafter incubated to effect enzymatic hydrolysis. Subsequently, the presence of the free 7-amino-4-trifluoro-methylquinolone is fluorometrically detected.

7-Amino-4-trifluoromethylquinolone derived substrates and method for

A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

Substrates and method for determining enzymes

Synthetic substrates are provided which may be represented as A-D. The A moiety thereof includes an amino acid, polypeptide, or derivative thereof. The D moiety thereof includes 7-amino coumarin derivatives having an electron withdrawing substituent group at the 3 position carbon or fused between the 3 and 4 position carbons.

Synthetic substrates for enzyme analysis

Growing community of inventors

Alamo, CA, United States of America

Eugene R Bissell