Vanderbilt University

Eugene J Leboeuf

David Edward Cliffel

Randall S Reiserer

John P Wikswo

Franz Baudenbacher

Frederick R Haselton

Mark Andrew Stremler

William Hudson Hofmeister

Lisa J McCawley

Ales Prokop

Charles P Lin

Chang Y Chung

Alex Prokop

John F Wikswo

A bioreactor () for cultivating living cells in a liquid medium. In one embodiment, the bioreactor () has a substrate () having a first surface and an opposite second surface, defining a channel () therein, and a plurality of chambers () formed in the substrate, wherein each of the plurality of chambers () is adapted for receiving cells in a liquid medium and formed with an open end (), an opposite closed end () and side walls, the open end () and the closed end () defining a depth, d, therebetween for the corresponding chamber (), the sidewalls defining a width, w, therebetween for the corresponding chamber (). As such formed, each chamber () is in fluid communication with the channel () through the open end of the chamber (), and at least two of the plurality of chambers () have depths or widths the same or different form each other.

Bioreactors with an array of chambers and a common feed line

A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.

Bioreactors with multiple chambers

A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.

Capillary perfused bioreactors with multiple chambers

Growing community of inventors

Franklin, TN, United States of America

Eugene J Leboeuf