Cornell University

Applera Corporation

Applied Biosystems, LLC

Roche Molecular Systems, Inc.

Unitaq Bio

Pe Corporation

Umitaq Bio

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Eugene G Spier

Francis Barany

Alain Mir

Yu Wang

Kenneth J Livak

Jingtao Sun

Ryan T Koehler

Marintha Heil

Francisco M De La Vega

Emily S Winn-Deen

Ellen H Fiss

Junko F Stevens

Jeremy Heil

Maureen Higgins

Ivy McMullen

Steven I Ladunga

Jeffery Fong

Slav Dugenny

Xiaoqing You

Lily Xu

Simon Greenberg

Leila G Smith

Michael Rhodes

Laurent R Bellon

Dawn Madden

Dennis A Gilbert

Karl J Guegler

Michael W Hunkapiller

Mark R Andersen

Victoria L Boyd

Kalyani Mangipudi

Christopher David Santini

Stephen A Glanowski

Chitra Manohar

Marcel Fontecha

Huan Truong

H Michael Wenz

Charles R Scaf

Nicolas Peyret

Susan Eddins

Michelle Elizabeth Yee

Thanh Tam

Ramani Sadanandam Ravirala

Julie Williams

Methods for the rapid detection of the presence or absence of SARS-CoV-2, influenza A and influenza B in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting SARS-CoV-2, influenza A, and influenza B and kits are provided that are designed for the detection of SARS-CoV-2, influenza A and influenza B.

Compositions and methods for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Influenza A and Influenza B

The present invention is directed to kits for identifying the presence of one or more target nucleotide sequences in a sample that involve a ligation and/or polymerase reaction. In some embodiments, the ligation products formed in the ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.

Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions

The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a ligation and/or polymerase reaction. In some embodiments, the ligation products formed in the ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.

Methods for the rapid detection of the presence or absence of Zika virus in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Zika virus and kits are provided that are designed for the detection of Zika virus.

Compositions and methods for detection of zika virus

Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.

Methods for placing, accepting, and filling orders for products and services

Compositions and methods for detection of Zika virus

The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.

The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more methylated or unmethylated target nucleotide sequences in the sample is identified based on the detection.

Method for relative quantification of changes in DNA methylation, using combined nuclease, ligation, and polymerase reactions

Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid. A variety of detection formats are provided, including solution-phase and surface-based formats. The methods and compositions are well-suited for highly multiplexed nucleic acid detection, and are applicable for the detection of any target nucleic acid of interest in both research and clinical settings.

Methods and compositions for universal detection of nucleic acids

Provided herein are methods and compositions for performing PCR with primers with blocked 3'-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.

Methods and compositions for PCR using blocked and universal primers

The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.

Methods, compositions, and kits for forming self-complementary polynucleotides

Methods and kits for detecting whether target nucleic acid sequences are present and/or quantitating target nucleic acid sequences are provided.

Methods for amplification of nucleic acids using spanning primers

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Palo Alto, CA, United States of America

Eugene G Spier