Tissue Engineering

Tei Biosciences, Inc.

Massachusetts Institute of Technology

Other

Organogenesis, Inc.

Aerojet-general Corporation

Eugene Bell

Timothy W Fofonoff

Vladimir Russakovsky

Tracy M Sioussat

Jianwu Dai

Paul D Kemp

Aslam A Malik

Marvin F Young

Boris D Nahlovsky

John Cavallaro

Louis Dubertret

Crispin Weinberg

Michael J Begley

Kathleen Regan

Valerie Mason

Lisa Falco

David T Kagan

A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.

EB matrix production from animal tissue and its use for tissue repair

A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The process includes, but is not limited to, harvesting the tissue, optionally extracting growth and differentiation factors from the tissue, inactivating infective agents of the tissue, mechanically expressing undesirable components from the tissue, delipidizing the tissue, washing the tissue, optionally drying the tissue, optionally cross-linking the tissue not necessarily in the order described. The resulting product, EBM, is characterized by its microbial, fungal, viral and prion inactivated state. EBM is strong, bioremodelable, drapable and does not undergo calcification. EBM supplants previous inventions because of its unique method of preparation and broad applicability in tissue reengineering.

Processing tissue to produce a biopolymer scaffold for tissue engineering

Cardiovascular components such as biocompatible heart valves and annular sewing rings are disclosed, as well as, methods for making the same. The heart valves include biodegradable polymer fiber scaffolds and collagen. Also disclosed are donor aortic heart valves processed without the use of crosslinking chemicals.

Cardiovascular components for transplantation and methods of making thereof

A method of forming and preserving a bioremodelable, biopolymer scaffold material is carried out by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The process may include, but is not limited to, harvesting the tissue, optionally extracting growth and differentiation factors from the tissue, inactivating infective agents of the tissue such as by treating with KOH or NaOH, mechanically expressing undesirable components from the tissue, delipidizing the tissue with organic solvents, mechanically expressing components released by the solvet, washing the tissue, optionally drying the tissue, and optionally cross-linking the tissue, not necessarily in the order described. The resulting product, named EB Matrix (EBM), is characterized by its microbial, fungal, viral and prion inactivated state. EBM is strong, bioremodelable, drapable and does not undergo calcification.

Processing fetal or neo-natal tissue to produce a scaffold for tissue engineering

Using animal tissues as starting materials, a method is described for producing extracellular matrix particulates. The invention includes an embodiment wherein the matrix particulates are applied to collagen scaffolds, which can be seeded with living cells or the particulates may alone be seeded with living cells. Further, the invention encompasses bonding the particulates to collagen foams, or collagen threads made into fabrics or to foams combined with threads. The particulates, with or without scaffolding, can be used as tissues for grafting or as model systems for research and testing. The invention also encompasses the spinning of threads on which the matrix particulates are components and the freeze drying of foams to whose surfaces the matrix particulates are attached.

Method and construct for producing graft tissue from an extracellular matrix

Apparatus and methods are disclosed for maturing an elongate replacement tissue construct in vitro prior to use of the replacement construct in vivo as, for example, a ligament. The tissue is seeded with specific cells, exposed to a maturation fluid, and subjected to selected forces, which can include longitudinal stress, (i.e. stressing the tissue along its elongate axis). The tissue is disposed in a maturation chamber that confines maturation fluid for introduction to the tissue. A first mounting element couples to a first end of the elongate biopolymer tissue and a second mounting element couples to a second end of the tissue such that the tissue extends along a longitudinal axis, and a force is applied to at least one of the mounting elements for longitudinally stressing the tissue. The foregoing apparatus and methods are intended to provide a replacement tissue that is more readily integrable in vivo, i.e., a tissue that more readily degrades, regenerates and remodels in vivo to produce a more durable and functional replacement tissue.

Methods and apparatus for the conditioning of ligament replacement tissue

Biopolymer matt, biopolymer matt composites, biopolymer matt compositions, and methods of preparing the matt and composite matts are described. Also described are biocompatible constructs which include extracellular matrix macromolecules and methods of preparing these constructs. The matt, matt compositions and biocompatible constructs of the invention can be used in tissue repair and reconstruction.

Biopolymer matt for use in tissue repair and reconstruction

Single and double density biopolymer foams, composite biopolymer foams including both single and double density foams, and methods of preparing these foams and composite foams are described. Also described are biocompatible constructs which include single or double density biopolymer foams and extracellular matrix particulates and methods of preparing these constructs. The foams, composite foams, and biocompatible constructs of the invention can be used in tissue repair and reconstruction.

Biopolymer foams for use in tissue repair and reconstruction

Method and construct for producing graft tissue from an extracellular

Method and apparatus are disclosed for removing biopolymer constructs from support structures used to impart a selected shape to the biopolymer. The biopolymer construct and the support structure are in intimate at an interface and adhere to each other. The support structure includes an endcap adapted for introducing a fluid to the interface for reducing adhesion between the biopolymer structure and the support structure by applying a separating force at the interface, thereby facilitating removal between the support structure and the biopolymer construct.

Method and apparatus for removal of biopolymer constructs from support

Methods for preparing biopolymer foams

Method for spinning and processing collagen fiber

Using connective tissues as starting materials, a method is described for producing matrix particulates. The invention includes an embodiment wherein the matrix particulate are seeded with living human cells or with cells of other species. Further, the invention encompasses fusing the particulates to constitute composites of various shapes, or holding them together in a porous container made of membranous biopolymers. The particulates or composites can be used as tissues for grafting or as model systems for research and testing. The invention also encompasses the spinning of threads in which the matrix particulates are components.

Growing community of inventors

Boston, MA, United States of America

Eugene Bell