Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Horiba Abx Sas

Horiba Abx Sas / Horiba Ltd

Estelle Gremion

Pierre Blandin

Anais Ali-Cherif

Aurelien Daynes

Olivier Cioni

Damien Isebe

Sebastien Raimbault

Aurélien Daynes

Sébastien Raimbault

The invention is a method for estimating a representative volume of particles of interest () immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (), the light source emitting an incident light wave () propagating towards the sample () along a propagation axis (Z); b) acquiring, by means of an image sensor (), an image (I) of the sample (), formed in a detection plane (P), the sample being arranged between the light source () and the image sensor (), each image being representative of a light wave () referred to as an exposure light wave, to which the image sensor () is exposed under the effect of illumination; c) using the image of the sample (I), acquired during step b), and a holographic propagation operator, to calculate a complex expression (A (x, y, z)) of the exposure light wave () in different positions relative to the detection plane; the method comprising a step of estimating the representative volume (AA) of the particles of interest () depending on the complex expressions calculated during step c).

Optical method for estimating a representative volume of particles present in a sample based on a size of each region of interest

The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Method for counting particles in a sample by means of lensless imaging

Growing community of inventors

La Tour du Pin, France

Estelle Gremion