Evolva Sa

International Flavors & Fragrances Inc.

River Stone Biotech LLC

Danstar Ferment Ag

University of Copenhagen

Esben Halkjaer Hansen

Michael Dalgaard Mikkelsen

Swee Chuang Lim Hallwyl

Jens Houghton-Larsen

Veronique Douchin

Simon Carlsen

Nina Nicoline Rasmussen

Philipp Friedrich Berninger

Manuel Quiros Asensio

Francesca Ambri

Adam Matthew Takos

Iben Nordmark Andersen

Jorgen Hansen

Joseph M Sheridan

Jonathan R Heal

William D O Hamilton

Joergen Hansen

Sabina Tavares

Charlotte Blom

Franziska Grassinger

Klavs Riishede Hansen

Helene Lunde Robertsen

Fanny Delgrange

Honey Polur

Jane Dannow Dyekjaer

Ganesh M Kishore

Paula M Hicks

Michael Naesby

Birger Lindberg Møller

Sathish Kumar

Joseph Michael Sheridan

Jon Richard Heal

Markus Schwab

Sabina De Andrade Pereira Tavares

Laura Tatjer Recorda

Michael Motion

Nethaji Janeshawari Gallage

Rubini Maya Kannangara

Jens Houghton Larsen

Sumire Honda Malca

Philipp Berninger

Angela Manuela Ribeiro De Carvalho

Jørgen Hansen

Helene Lunde Robertson

Polur Honey

William DO Hamilton

Murali Panchapagesa

Kalyan K Kumar

Birger Lindberg Moller

Simon Ernesto

Tange Thomas Ostergaard

Shailendra Sonkar

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

Recombinant production of steviol glycosides

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

Production of steviol glycosides in recombinant hosts

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors.

Increased biosynthesis of benzylisoquinoline alkaloids and benzylisoquinoline alkaloid precursors in a recombinant host cell

The invention relates to recombinant host cells that expresses one or more genes encoding a cytochrome P450 enzyme capable of N-demethylating and/O-demethylating reticuline and/or derivatives thereof, and also methods of producing a N-demethylated and/or O-demethylated reticuline and/or derivatives thereof, comprising cultivating the recombinant host of the invention in a culture medium under conditions in which the one or more genes encoding the cytochrome P450 enzymes is/are expressed. The reticuline and derivatives thereof are useful for providing access to naturally unavailable and chemically difficult-to-produce starting materials for opioids.

Demethylation of reticuline and derivatives thereof with fungal cytochrome P450

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.

Compositions and methods for the biosynthesis of vanillin or vanillin beta-d-glucoside

The invention relates to methods for producing vanillin and related compounds. The methods involve use of a vanillin synthase capable of catalyzing side chain cleavage of ferulic acid to form vanillin. The invention also relates to host organisms expressing such vanillin synthases useful in the methods.

Vanillin synthase

Production of steviol glycosides in microorganisms

Compositions and methods for the biosynthesis of vanillan or vanillin beta-D-glucoside

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.

Compositions and methods for the biosynthesis of vanillin or vanillin beta-D glucoside

Recombinant host cell for the biosynthesis of vanillin or vanillin beta-D-glucoside

Growing community of inventors

Frederiksberg, Denmark

Esben Halkjaer Hansen