Novozymes A/s

Novo Nordisk A/s

Erwin Ludo Roggen

Arne Agerlin Olsen

Claus Von Der Osten

Tine Muxoll Fatum

Kim Vilbour Andersen

Steffen Ernst

Heinz-Josef Deussen

Heinz Josef Deussen

The properties of a fungal lipolytic enzyme can be altered by substituting amino acid residues corresponding to certain specified amino acid residues in thelipase. The altered property may be, e.g., an increased thermostability, an altered pH dependence, or an altered substrate specificity.

Lipolytic enzyme variant

A polypeptide-polymer conjugate is prepared having one or more polymers covalently bound to a polypeptide. The polymer has the general formula; EO PO wherein EO is ethylene oxide, PO is propylene oxide, x&equals;1&minus;99%, y&equals;1&minus;99%, x&plus;y&equals;100%, and x and y are in a ratio range of 10:90 to 90:10, more preferably 40:60 to 60:40, or in a 50/50 ratio. The polymer, which can be a block polymer, typically has a molecular weight in a range of 100 to 100,000 daltons, and the polypeptide may contain 1 to 100 polymer molecules. Coupling the polypeptide, in particular enzymes, to the polymer reduces respiratory allergenicity of the polypeptide. Industrial compositions such as detergent compositions, personal care compositions, compositions for processing/treating textiles, and food and feed compositions containing the polypeptide-polymer conjugate have reduced respiratory allergenicity.

Polypeptides conjugated to copolymers of ethylene oxide and propylene oxide to reduce allergenicity

The present invention relates to polypeptide-polymer conjugates having added and/or removed one or more attachment groups for coupling polymeric molecules on the surface of the polypeptide structure, a method for preparing polypeptide-polymer conjugates of the invention, the use of said conjugates for reducing the immunogenicity and allergenicity and compositions comprising said conjugate.

Modified enzymes having polymer conjugates

The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.

Modified polypeptide

The present invention relates to modified polypeptides with reduced respiratory allergenicity having polymeric molecules with a molecular weight from 100 up to 750 Da, coupled covalently to the parent polypeptide having a molecular weight from 5 to 100 kDa. The present invention also relates to industrial compositions comprising modified polypeptide with reduced respiratory allergenicity, skin care products, the use of modified polypeptides for reducing the allergenicity of industrial composition and products and finally a method for reducing the allergenicity of polypeptides.

Polypeptides conjugated with polymers

The present invention relates to polypeptide-polymer conjugates having added and/or removed one or more attachment groups for coupling polymeric molecules on the surface of the polypeptide structure, a method for preparing polypeptide-polymer conjugates of the invention, the use of said conjugated for reducing the immunogenicity and allergenicity and compositions comprising said conjugate.

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Lyngby, Denmark

Erwin Ludo Roggen