University of Miami

Ervin Essenfeld

Azorides Morales

Harold Essenfeld

Harold D Kimrey, Jr

A process and apparatus for rapid, continuous flow histological processing of tissues is disclosed. The steps of fixation, dehydration, clearing and impregnation are performed in less than one hour; this allows a pathologist to evaluate samples shortly after receipt, perhaps while the patient is still in the operating room. Rapid and continuous processing is accomplished by decreasing the thickness of tissue sections, use of non-aqueous solutions composed of admixtures of solutions, solution exchange at elevated temperature and with agitation, and impregnation under vacuum pressure. The patient in surgery is thus provided with point-of-care surgical pathology.

High quality, continuous throughput, tissue processing

A process and apparatus for rapid, continuous flow histological processing of tissues is disclosed. The steps of fixation, dehydration, clearing and impregnation are performed in less than one hour; this allows a pathologist to evaluate samples shortly after receipt, perhaps while the patient is still in the operating room. Rapid and continuous processing is accomplished by decreasing the thickness of tissue sections, use of nonaqueous solutions composed of admixtures of solutions, solution exchange at elevated temperature and with agitation, and impregnation under vacuum pressure. The patient in surgery is thus provided with point-of-care surgical pathology.

An improved microwave unit and tissue processor system incorporating the unit are provided for use in rapid tissue processing. The microwave unit may be comprised of an energy source, a waveguide transmitting the microwave energy to a reaction chamber, and the reaction chamber being adapted to process tissue specimens for histology. The unit provides gentle and uniform heating, with minimal heat loss and escape of volatile chemicals. The system may be operated continuously and/or batchwise, by manual operation or automatically. The automated system may be operated with continuous throughput to obtain the advantages of the invention such as, for example, rapid processing under two hours and/or preservation of cell structure and tissue architecture.

Rapid tissue processor

Apparatus for high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation

A grossing board for preparing gross tissue samples having relatively uniform thickness features one or more tissue-receiving depressions and one or more knife-guiding members. The bottom surface of a tissue-receiving depression is essentially parallel to the upper, cutting surface of the grossing board, and the knife-guiding members are configured to guide the pathologist's knife so as to ensure cuts that are parallel to the bottom surface of the tissue-receiving depression, thereby yielding gross tissue samples of desired reproducible, uniform thickness.

Growing community of inventors

Caracas, Venezuela

Ervin Essenfeld