Boehringer Ingelheim International Gmbh

Genentech, Inc.

The University of North Carolina at Chapel Hill

Other

Boehringer Ingelheim Gmbh

Genetech, Inc.

Ernst Wagner

Matthew Cotten

Max L Birnstiel

David T Curiel

Kurt Zatloukal

Berndt Oberhauser

Christian Plank

Walter G Schmidt

Hartmut Beug

Michael Buschle

Wolfgang Zauner

Susanna Chiocca

Gotthold Schaffner

Harald Kandolf

Walter G M Schmidt

Ping-chuan Hu

Genetics units for inhibiting the function of RNA

A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

Composition for introducing nucleic acid complexes into higher eucaryotic cells

Composition for introducing nucleic acid complexes into higher

Composition comprising an endosomolytic agent for introducing nucleic

Method for introducing nucleic acids into higher eukaryotic cells

Complexes containing nucleic acid which can be taken-up by endocytosis

The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed with the polyanionic nucleic acid. This complex is bound to the transferrin receptor, which is highly regulated in growing cells, and absorbed into the cell. Suitable nucleic acids include those which inhibit specific genes or the RNA function, such as antisense oligonucleotides or ribozymes or the genes coding for them. The invention further relates to a process for introducing nucleic acids into the cells, transferrin-polycation/nucleic acid complexes and pharmaceutical preparations containing them.

Protein-polycation nucleic acid complexes and methods of use

Endosomolytically active particles

Adenovirus for delivering foreign DNA into higher eukaryotic cells

Conjugates in which a virus is bound via an antibody to a substance having an affinity for nucleic acid, for transporting gene constructs into higher eucaryotic cells. Complexes of the conjugates and nucleic acid are internalized in the cell, whilst the virus as part of the complex brings about the internalization and the release of the contents of the endosomes, in which the complexes are located after entering the cell. Pharmaceutical preparations in which the nucleic acid is a therapeutically active gene construct, particularly for use in gene therapy.

Conjugates for introducing nucleic acid into higher eucaryotic cells

The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed with the polyanionic nucleic acid. This complex is bound to the transfertin receptor, which is highly regulated in growing cells, and absorbed into the cell. Suitable nucleic acids include those which inhibit specific genes or the RNA function, such as antisense oligonucleotides or ribozymes or the genes coding for them. The invention further relates to a process for introducing nucleic acids into the cells, transferrin-polycation/nucleic acid complexes and pharmaceutical preparations containing them.

Protein-polycation conjugates

Growing community of inventors

Langenzersdorf, Austria

Ernst Wagner