Systemix, Inc.

Ernst Bohnlein

Richard J Rigg

Sean P Forestell

Ivan Plavec

Jonathan S Dando

Jingyi Chen

Method for obtaining retroviral packaging cell lines producing high

This invention provides a method for obtaining a recombinant retroviral packaging cell capable of producing retroviral vectors and the recombinant packaging cell obtained by the method. Also provided is a method of producing recombinant retroviral particles obtained by introducing into the packaging cells obtained according to the methods disclosed herein, a recombinant retroviral vector and propagating the resulting producer cells under conditions favorable for the production and secretion of retroviral vector supernatant. The retroviral supernatants produced by these methods also is claimed herein. This invention further provides a method for screening retroviral vector supernatant for high transduction efficiency and methods for producing retroviral vector supernatant for transducing cells with high efficiency in gene therapy applications.

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Los Altos, CA, United States of America

Ernst Bohnlein