Chiron Corporation

University of California

Cetus Corporation

Cetus Oncology Corporation

Hoffmann-la Roche Inc.

Roche Molecular Systems, Inc.

Ernest S Kawasaki

Martha B Ladner

Mazie Yee Coyne

Kirston E Koths

Robert F Halenbeck

George A Martin

Janelle A Noble

Janelle N Van Arsdell

Robert E Sobol

Mark R Green

Peter Ralph

David F Mark

Alice M Wang

Francis P McCormick

Michael V Doyle

Mary Kim Warren

Michael P McGrogan

Owen O Witto

A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system, is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both &ldquo;long&rdquo; and &ldquo;short&rdquo; forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.

DNA encoding N&nabla;2 CSF-1 (short form) and carboxy truncated fragment thereof

A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system. is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both 'long' and 'short' forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.

Point mutants of N.gradient.2 CSF-1 and carboxy truncated fragments

A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both 'long' and 'short' forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.

DNA encoding NV2 (long form) and carboxy truncated fragments thereof

N.gradient.2-CSF-1(long form) and carboxy truncated fragments thereof

N.gradient.2 CSF-1 (short form) and carboxy truncated fragments thereof

Methods are provided for detecting carcinoma metastases in selected body tissues and fluids. These methods offer greater than 1,000-fold enhanced sensitivity compared to prior standard diagnostic methods. In one embodiment of the invention, target carcinoma associated nucleic acid sequences are identified for detecting minimal residual disease in lung carcinomas. The methods utilize nucleic acid amplification techniques, preferably, the polymerase chain reaction.

Detection of carcinoma metastases by nucleic acid amplification

DNA for new forms of CSF-1 are provided which relate to N-terminal and C-terminal truncations. Specifically, N-.gradient.3 terminal truncations of the short and long forms of CSF-1 have been found to be particularly advantageous. These forms may have a C-terminal truncation at a variety of different amino acids beyond position 149.

DNA encoding N- and C- terminally truncated colony stimulating factor-1

New forms of CSF-1 are provided which relate to N-terminal truncations. Specifically, N-.gradient.3 terminal truncations of the long form of CSF-1 have been found to be particularly advantageous. These forms may have a C-terminal truncation at a variety of different amino acids beyond position 150.

N- 3 deletion mutants of the long form of CSF-1

New forms of CSF-1 are provided which relate to N-terminal truncations. Specifically, N-.gradient.3 terminal truncations of the short form of CSF-1 have been found to be particularly advantageous. These forms may have a C-terminal truncation at a variety of different amino acids beyond position 150.

N-.gradient.3 deletion mutants of the short form of CSF-1

DNA encoding various forms of colony stimulating factor-1

A colony stimulating factor. CSF-1, is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein.

Recombinant colony stimulating factor-1

The invention provides highly sensitive methods for detecting specific sequences contained in chimeric mRNA. The mRNA sequences are reverse transcribed into complementary DNA (cDNA), amplified by the Polymerase Chain Reaction, and detected by hybridization with a labeled sequence specific oligonucleotide probe. The method is particularly valuable for the detection of chimeric mRNAs experessed by activated oncogenes that result from aberrant genetic rearragements such as chromosomal translocations.

Chimeric messenger RNA detection methods

DNA sequences and recombinant DNA molecules which encode human IL-2 IL-2 like polypeptides, hosts transformed with vectors carrying the DNA sequences as inserts, methods for their production and therapeutic formulations utilizing the IL-2 and IL-2 like polypeptides are provided.

Growing community of inventors

Richmond, CA, United States of America

Ernest S Kawasaki