Ortho Diagnostic Systems Inc.

Warner-lambert Company

Ernest G Schutt

Henry A Graham, Jr

Houston G Brooks, Jr

Utpal R Chakraborty

Chi-Deu Chang

Jennifer A Nasser

David L Greenwood

Sharon R Lawler

Lloyd L Hollenbeck, Jr

Karin L Utberg

Albert Venturini

Richard S Dondero

George B Hovorka

William P Hansen

Raymond E Meyer

A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

Kit containing glutaraldehyde coated colloidal metal particles of a

Method of producing a metal sol reagent containing colloidal metal

A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio.

Scattered total internal reflectance immunoassay system

A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.

Cuvette for receiving liquid sample

A method is disclosed for preparing an activated microporous substrate to which antibody may be covalently bonded through trichloro-triazine to yield an activated substrate which is very useful for many immunochemical reactions utilizing a flow-through procedure.

Substrate for immunoassay and means of preparing same

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Long Valley, NJ, United States of America

Ernest G Schutt