Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Enchi Corporation

Mascoma Corporation

Universiteit Stellenbosch

Erin Wiswall

John E McBride

Mark Mellon

Elena E Brevnova

Anne K Warner

Chhayal V Gandhi

Aaron Argyros

Charles F Rice

Ryan Skinner

Emily Stonehouse

Haowen Xu

Heidi H Hau

Kevin S Wenger

Indraneel Shikhare

Trisha Barrett

Nicky Caiazza

Vineet Rajgarhia

Allan C Froehlich

Riaan Den Haan

Shital A Tripathi

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Alan Belcher

Vineet B Rajgarhia

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

John Mcbride

John E Mcbride

Arthur J Shaw, Iv

Brooks Henningsen

David Anthony Hogsett

Michelle Oeser

Janet Fisher

Emile Van Zyl

J Kevin Kraus

Shaunita Hellouise Rose

Danie Lagrange

Naomi Thorngren

Chris Herring

Elena Brevnova

Johannes Van Eijk

Heidi Lau

Shaunita Rose

Abigail S Foster

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Cell-associated heterologous food and/or feed enzymes

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by, orin host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of termite cellulases in yeast

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Expression of enzymes in yeast for lignocellulose derived oligomer CBP

The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Yeast cells expressing an exogenous cellulosome and methods of using the same

Thermophilic gram-positive anaerobic host cells, for example(''), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, ahost cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Heterologous biomass degrading enzyme expression in thermoanaerobacterium saccharolyticum

Heterologous expression of termite cellulases yeast

Growing community of inventors

Danbury, NH, United States of America

Erin Wiswall