Becton Dickinson and Company Limited

Erin Gooch Carruthers

Dwight Livingston

Melody M H Kuroda

Artis R Lockhart

Adam C Curry

Kristin Weidemaier

Eric Fallows

Ernie Ritchey

Spencer Lovette

Scott N Danhof

Miroslaw Bartkowiak

Jack Kessler

Robert L Campbell

Mark A Talmer

Thomas D Haubert

Jack H Kessler

Ammon David Lentz

James A Prescott

Michael L Marshall

Gregory S Kramer

Michael Justin Lizzi

Scott Nicholas Danhof

M Scott Ulrich

Andrea Liebmann-Vinson

Randy J Somerville

Kevin G Dolan

David S Sebba

Donald E Gorelick

Wendy Dale Woodley

Jeffrey S Ojala

Eric A Fallows

Melody MH Kuroda

Melody Mh Kuroda

David S Sebra

Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Methods, systems, and devices for detecting and identifying microorganisms in microbiological culture samples

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Cary, NC, United States of America

Erin Gooch Carruthers