Codexis, Inc.

Buzzbite, LLC

Merck Sharp + Dohme Corp.

Erika M Milczek

Xiyun Zhang

Jeffrey C Moore

Vesna Mitchell

Robert Osborne

Khin Yu Naing Htwe

Jie Yang

Goutami Banerjee

Melissa Ann Mayo

Stephan Jenne

Jovana Nazor

Vinay M Girijavallabhan

James Nicholas Riggins

Zhiguo Jake Song

Edward Cleator

Michael J Williams

Andrew William Gibson

Stephen Philip Keen

David R Lieberman

Feng Peng

Alejandra Garcia Earley

Jongrock Kong

Alejandro Dieguez Vazquez

Mark Mclaughlin

Kevin M Belyk

Khin Yu Htwe

The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

Penicillin-G acylases

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

P450-BM3 variants with improved activity

The present invention provides engineered transglutamirase enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, methods of producing these enzymes, and methods of using the engineered transglutaminase enzymes.

Transglutaminase variants

Variant penicillin-G acylases

Topical formulations of proteases, devices including the formulations are disclosed. Described herein are packages for storing and dispensing multi-part topical enzyme formulations and neutralizing agent, wherein the contents of the parts are mixed, and the enzyme is taken into solution containing a neutralizing agent (skin protectant) for treatment of skin abrasions and disorders. Particular multi-part topical formulation disclosed here relates to immediate (neutralizing agent) and extended relief (enzymes) from itching and inflammation associated with insect stings, insect bites, contact dermatitis such as poison ivy and poison oak, and eczema. The formulations and packaging using this principle and methods of use thereof are also provided. Additionally this invention contains active ingredients for wound cleaning and debridement.

Enzyme formulations, devices and methods of use

Growing community of inventors

New York, NY, United States of America

Erika M Milczek