Alligator Bioscience Ab

Erika Gustafsson

Christina Furebring

Karin Haraldsson

Björn Walse

Anna Rosén

Johannes A G Van Strijp

Cornelis P M Van Kessel

Petrus J A Haas

Lena Schultz

The present invention provides a polypeptide having a biological activity of the Chemotaxis Inhibitory Protein of('CHIPS'), the polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 2, or a fragment or variant thereof having a biological activity of CHIPS, wherein the fragment or variant retains amino acid substitutions K40E, D42V, N77H, K100R, K105R, N111 K and/or G112A relative to the wildtype CHIPS protein of SEQ ID NO:1. In one embodiment, polypeptide consists of the amino acid sequence of SEQ ID NO: 2. Related aspects of the invention provide pharmaceutical compositions comprising a polypeptide of the invention, together with methods or making and using the same.

Polypeptides and use thereof

The present invention provides a polypeptide having a biological activity of the Chemotaxis Inhibitory Protein of('CHIPS'), the polypeptide comprising a variant of the amino acid sequence of SEQ ID NO:1. Preferably, the polypeptide is a CHIPS variant wherein one or more of the following amino acids is modified: N31, S32, G33, L34, P35, K40, D42, R46, Y48, K50, G52, T53, K54, N55, S56, A57, Q58, K61, E67, K69, L76, N77, P79, D83, L90, K92, K100, K101, S104, K105, S107, Y108, N111 and G112. In a preferred embodiment, the polypeptide is less immunogenic hi humans than the wildtype CHIPS protein. The invention further provides methods of making and using such variant CHIPS polypeptides.

Growing community of inventors

Bjarred, Sweden

Erika Gustafsson