Ab Kabi

Kabi Ab

Erik Yngve Holmer

Lars-Olov Andersson

Gudrun E Backstrom

Ulf P Lindahl

John Y Thunberg

Lars-Ake Fransson

Ewa G Soderstrom

Inga H Sandberg

Heparin fragments having selective anticoagulation activity having 14-18 sugar units, the disaccharide unit L-iduronosyl-2-O-sulphate-N-sulpho-U-glucosamine-6-O-sulphate being the main component, and where unsulphated L-iduronic acid is in a position situated 3-5 sugar units from the unreducing terminal. Pharmaceutical compositions containing such heparin fragments. Processes for the preparation of the heparin fragments.

Heparin fragments having selective anticoagulation activity

Crude clinically used heparin preparations having a low specific activity, for example, about 130 heparin units per milligram are purified by contacting such liquid crude heparin preparations with a gel matrix complexed with (i.e. having linked to it) antithrombin or inter-alpha-trypsin inhibitor as a heparin-binding plasma protein, and then desorbing or eluting the heparin from the antithrombin or inter-alpha-trypsin inhibitor complexed gel matrix as with an applicable buffer, removing any salt in the solution with the heparin by gel filtration and then drying the heparin as by lyophilization.

Heparin purification method

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Bromma, Sweden

Erik Yngve Holmer