University of New Mexico

Science & Technology Corporation &commat; Unm

Erik S Wallen

Pope L Moseley

Jan Roigas

A method for purifying heat shock protein complexes is provided which comprises the steps of adding a solution containing heat shock protein complexes, in which heat shock proteins are associated with peptides, polypeptides, denatured proteins or antigens, to a column containing an ADP matrix to bind the heat shock proteins complexes to the ADP matrix and adding a buffer containing ADP to the column to remove the heat shock protein complexes in an elution product. Additionally a method for synthesizing heat shock protein complexes and purifying the complexes so product is provided which comprises the steps of adding heat shock proteins to an ADP matrix column to bind them to the matrix, adding a solution of peptides, polypeptides, denatured proteins or antigens to the column to bind them to the heat shock proteins as heat shock protein complexes and adding a buffer containing ADP to the column to remove the complexes in all elution product.

Purified heat shock protein complexes

The present invention provides a heat shock protein immunotoxin comprising: at least a fragment of a heat shock protein, the fragment being capable of being bound by an immune cell; and a toxin bound to the fragment. The present invention also provides a method for decreasing the number of immune cells in an individual using the heat shock protein immunotoxins of the present invention. In addition, the present invention provides a method for decreasing the number of immune cells in an organ using the heat shock protein immunotoxins of the present invention.

Methods for using heat shock proteins

A method for purifying heat shock protein complexes is provided which comprises the steps of adding a solution containing heat shock protein complexes, in which heat shock proteins are associated with peptides, polypeptides, denatured proteins or antigens, to a column containing an ADP matrix to bind the heat shock proteins complexes to the ADP matrix and adding a buffer containing ADP to the column to remove the heat shock protein complexes in an elution protein. Additionally a method for synthesizing heat shock protein complexes and purifying the complexes so produced is provided which comprises the steps of adding heat shock proteins to an ADP matrix column to bind them to the matrix, adding a solution of peptides, polypeptides, denatured proteins or antigens to the column to bind them to the heat shock proteins as heat shock protein complexes and adding a buffer containing ADP to the column to remove the complexes in an elution product.

A method for purifying heat shock protein complexes is provided which comprises the steps of adding a solution containing heat shock protein complexes, in which heat shock proteins are associated with peptides, polypeptides, denatured proteins or antigens, to a column containing an ADP matrix to bind the heat shock proteins complexes to the ADP matrix and adding a buffer containing ADP to the column to remove the heat shock protein complexes in an elution product. Additionally a method for synthesizing heat shock protein complexes and purifying the complexes so produced is provided which comprises the steps of adding heat shock proteins to an ADP matrix column to bind them to the matrix, adding a solution of peptides, polypeptides, denatured proteins or antigens to the column to bind them to the heat shock proteins as heat shock protein complexes and adding a buffer containing ADP to the column to remove the complexes in an elution product.

The present invention provides a method for synthesizing heat shock protein-peptide complexes comprising the steps of: adding a shock protein to a denatured protein matrix to bind the heat shock protein to the denatured protein matrix; and adding a complexing solution comprising a peptide to elute a heat shock protein-peptide complex. The present invention also provides a heat shock protein-peptide complex synthesized by the method of the invention. In addition the present invention provides an apparatus for synthesizing heat shock protein-peptide complexes comprising a heat shock protein complex bound to a denatured protein matrix.

Growing community of inventors

Albuquerque, NM, United States of America

Erik S Wallen