Koninklijke Philips Corporation N.v.

Erik Robbert Vossenaar

Mark Thomas Johnson

Bernardus Hendrikus Wilhelmus Hendriks

Stein Kuiper

Hendrik Roelof Stapert

Nijs Cornelis Van Der Vaart

Gerhard Spekowius

Marc Wilhelmus Gijsbert Ponjee

The invention relates to a method of determining in vitro the amount of nuclear DNA within a human or animal cell using UV absorption measurement with UV light. The invention also relates to a method for detecting in vitro cancerous cells in a biological sample relying on the aforementioned principles. The invention also relates to an in vitro method of diagnosing or predicting the likely occurrence of cancer in a human or animal subject.

Vitro method of detecting and/or diagnosing cancer using UV light based DNA image cytometry

The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants () are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant () and/or the target substance () comprises a label () with an observable property, like fluorescence, that changes if the target substance () is bound to the reactant (), said property being detected by an array of sensor elements, for example photosensors (SE). The fluorescence of the label () may preferably be transferred by FRET to a different fluorescent label () or quenched if the target substance () is bound.

Microelectronic sensor device for DNA detection

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De Meern, Netherlands

Erik Robbert Vossenaar