Novartis Finance Corporation

Syngenta Participations Ag

Syngenta Investment Corporation

Ciba-geigy Corporation

Novartis Corporation

Queensland University of Technolgy

Syngenta Investments, Inc.

Erik M Dunder

Michael G Koziel

Nalini M Desai

Gregory W Warren

Martha S Wright

Gary M Pace

Karen L Launis

John L Dawson

Kelly S Lewis

Stephen V Evola

Janet L Suttie

Cindy G Bowman

Steven J Rothstein

Lyle Dean Crossland

Ellis J Merlin

Nadine B Carozzi

Vance C Kramer

Vance Cary Kramer

Moez Rajabali Meghji

Weining Gu

Heng Zhong

Yin-Fu Chang

Eric Boudreau

Annick Jeanne De Framond

Bruce Wayne Skillings

Paulo Cezar De Lucca

Robert L Miller

Robert Jason Christopher Geijskes

James O Linder

Manuel Benito Sainz

Christopher P Clucas

Cindy Boyce

Francis P Cammack

Jan Suttie

Francis E Cassagne

Steve Evola

Alan W Mousel

Shujie Dong

Albert R Hornbrook

Andreas H Tanner

Gilles Pollini

Sabrina Rouse

Terry Ray Colbert

The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with aninoculation suspension, saidcomprising a nucleic acid of interest, to obtain an-inoculated sugar cane tissue or cell thereof; b) co-cultivating said-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.

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Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash,and pepper.

Methods for stable transformation of plants

DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from . Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

Transgenic maize seed and method for controlling insect pests

Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.

Method of producing transgenic maize using direct transformation of commercially important genotypes

DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from,. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

Synthetic DNA sequences having enhanced insecticidal activity in maize

DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

Synthetic DNA sequence having enhanced insecticidal activity in maize

Nucleic acid promoter fragment isolated from a plant tryptophan synthase

Synthetic DNA sequence having enhanced activity in maize

Growing community of inventors

Chapel Hill, NC, United States of America

Erik M Dunder