Gist-brocades B.v.

Dsm N.v.

Cosmoferm B.v.

Dsm Patents & Trademarks

Dms N.v.

Erik De Vroom

Jan Metske Van Der Laan

Jan Verweij

Andreas Hoekema

Roelof Ary Bovenberg

Bertus Pieter Koekman

Roelof Ary Lans Bovenberg

Dirk Schipper

Adrianus Wilhelmus Hermanus Vollebregt

Maarten Nieboer

Johannis Lugtenburg

Thomas Van Der Does

John Krijgsman

Pieter Gijsbert Weber

Herman Pieter Fasel

Henri&euml;tte Elisabeth Anna De Braal

Jan Willem Hubert Smeets

The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.

Process for the fermentative production of deacylated cephalosporins

The present invention discloses an improved process for the N-acylation of amino alcohols employing an organic acid in the form of an acid halogenide, wherein the acylation occurs in an organic solvent in the additional presence of water.

Process for the acylation of amino alcohols

The present invention disclosures a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.

The present invention relates to a novel process for the preparation of cephalosporins having been deacylated at the 7-amino group, by fermentation of a cephalosporin producing microorganism in the presence of a side chain precursor, extraction of the N-substituted cephalosporin compound as present in the fermentation broth or fluid to an organic solvent, back extraction of the N-substituted cephalosporin compound to water, treatment of the aqueous phase with a dicarboxylate acylase and isolation of the crystalline cephalosporin compound according to formula (1) from the conversion solution, characterized in that the fermentation broth or fluid is incubated at acidic conditions and an elevated temperature prior to extraction of the N-substituted cephalosporin compound to an organic solvent. Further improvements of the process are obtained by washing the first organic solvent extract with acidified water and/or by extraction of the side chain to an organic solvent and/or by treating an aqueous cephalosporin solution produced at one or more stages in the process of the invention with carbon dioxide.

Process for the fermentative production of cephalosporin

A process to prepare the E isomer of a 3-substituted (6R,7R)-7-phenyl-acetamido-ceph-3-em-4-carboxylic acid from a mixture of the E and Z-isomer of either the corresponding tert-butyl or the corresponding 4-methoxybenzyl ester of a 3-substituted (6R,7R)-7-phenylacetamido-ceph-3-em-4-carboxylate, characterized by the application of a compound selected from the group titanium tetrachloride, tin tetrachloride and tellurium tetrachloride.

De-esterification process

Penicillin G acylase immobilized with a crosslinked mixture of gelled

Process for the efficient production of 7-ADCA via 3-(carboxyethylthio)

Process for the efficient production of 7-ADCA via

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Leiden, Netherlands

Erik De Vroom