Incyte Genomics, Inc.

Erik Bjeldanes

Lyle J Arnold

Tod Bedilion

Douglas Gilliland

Loren D Titsworth

Thomas P Theriault

Warren Lei

Steve Daniel

Lozen Titsworth

A method and apparatus is provided for mixing a film of fluid, particularly a film of chemical, biochemical, or biological fluids, undergoing a reaction. The apparatus comprises a means for creating a bubble within the film of fluid, and moving the bubble in the fluid, thereby mixing the fluid.

Microarray hybridization chamber

Methods are provide for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.

Normalization control for hybridization reactions

The invention provides methods and compositions for amplifying RNA sequences by (a) hybridizing to a target RNA a first primer comprising a 3&prime; target RNA hybridizing sequence and a first 5&prime; defined amplifyable sequence; (b) extending the first primer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the first cDNA strand a second primer comprising a 3&prime; random cDNA hybridizing sequence and a second 5&prime; defined amplifyable sequence; (d) extending the second primer with a DNA polymerase to form a second cDNA strand; and (e) amplifying the second cDNA strand with a third primer comprising the first 5&prime; defined amplifyable sequence.

Single primer PCR amplification of RNA

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Lafayette, CA, United States of America

Erik Bjeldanes