Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Novilux, LLC

Erich Kyger

William E Ortyn

Richard A Livingston

Jenny A Johnson

Kurt Weindel

Peter Wenzig

Judith Pinsl-Ober

Knut Bartl

Ralf Schoenbrunner

Patrick O'Donnell

Khushbeer Malhotra

Robert Onorato

Brian W Noland

John David Jolly

Jeffrey J Bishop

Danni Wang

Michele Gilbert

Luis H Monsalve

The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.

Recovery of nucleic acids from magnetic glass particles

This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.

Use of silica material in an amplification reaction

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Antioch, CA, United States of America

Erich Kyger