Pioneer Hi-bred International, Inc.

Erica Unger

Andrew Mark Cigan

Yongzhong Wu

Howard P Hershey

Timothy W Fox

Tim W Fox

Andrew M Cigan

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, methods for testing allelic variants, and methods for repressing transmission of transgenes.

Dominant gene suppression transgenes and methods of using same

Methods are provided in which a promoter hairpin construct is introduced into a cell to suppress expression of a gene of interest; a change in phenotype of the cell, or of an organism regenerated from the cell, is detected; and the change in phenotype identifies the function of a product of the gene of interest. Also provided are methods for restoring the original phenotype.

Method for identifying gene function

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of such plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of plants generated or maintained by the methods, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, and methods for repressing transmission of transgenes.

Growing community of inventors

Ames, IA, United States of America

Erica Unger