Cis Bio International

Centre National De La Recherche Scientifique

Cisbio Bioassays

Ion Beam Applications S.a.

Eric Trinquet

Gérard Mathis

Hervé Bazin

Jurriaan Zwier

Michel Fink

Laurent Lamarque

Emmanuel Bourrier

Hervé Ansanay

Norbert Tinel

Michel Laget

Gerard Mathis

Jean-Philippe Pin

Iris Pribilla

G&eacute;rard Mathis

Elodie Julie Dupuis

Marc Preaudat

Achim Brinker

Thomas Roux

Patrizia Alberti

Sraboni Ghose

Emmanuel Claret

Jean-Phillippe Pin

Etienne Doumazane

Thierry Durroux

Herve Ansanay

Robert Poole

The invention relates to compounds of formula: in which X, Y, L and Lnare as defined in the description.

Fluorescent GTP analogues and use

The invention relates to compounds of formula (I'): in which A, L2, M and B are as defined in the description. These compounds are substrates of O6-alkylguanine-DNA alkyltransferase and mutants thereof.

Substrates of O6-alkylguanine-DNA alkyltransferase and mutants thereof

The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.75 V; or (3) an agent which binds specifically, by non-covalent bonding, with the fluorescent metal complex; (d) adding a metal ion which competes with the rare earth so as to form a non-fluorescent metal complex; (d) measuring the luminescence emitted by the reaction medium at the emission wavelength of the fluorescent metal complex and/or at the emission wavelength of the modulating compound when the compound is a fluorescent acceptor compound; and (e) comparing the signal measured in step d) with a reference signal measured on cells having been subjected only to steps a) and c).

Method for detecting membrane protein internalization

The invention relates to a method for selecting compounds having a modulating effect on the activation state of a dimer of VFT-domain proteins expressed in cell membranes present in a measuring medium, said dimer consisting of a first protein and of a second protein, said proteins being identical or different, wherein this method comprises the following steps: The invention can be used in the search for new medicaments and new taste modulators.

Method for detecting compounds modulating dimers of VFT domain membrane proteins

The invention relates to a method for determining whether a test compound binds preferentially to a membrane receptor R1 or to a membrane receptor R2, these receptors being known to be expressed on the surface of the cells in monomeric, homodimeric or heterodimeric form. This method is applied using one or two FRET partner pairs.

Method for determining the binding of a given compound to a membrane receptor

Method for the detection in homogeneous medium of a post-translational modification of a proteinaceous substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the proteinaceous substrate and a first coupling domain and in that it comprises the following stages:

Method for the detection of post-translational modifications

The invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules in living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair.

Method for detecting intracellular interaction between biomolecules

The invention relates to a method for suppressing the FRET emitted by a reaction medium containing a pair of fluorescent FRET partner conjugates specific for a biological event, characterized in that a FRET signal killer which does not disturb said biological event is introduced into this medium.

Method for suppressing a FRET signal, FRET signal suppressor agents and use in a method for multiplexing biological events

The present invention relates to inositol phosphate derivatives, in which the inositol phosphate is substituted with one or two reactive groups G or one or two conjugated substances or molecules M, said reactive group(s) G or said substance(s) or molecule(s) M being linked to IP1 via a linkage group L, M being chosen from the following group: a tracer, an immunogen, a member of a binding partner pair, a solid support. Application: tools allowing the study of the inositol phosphate cycle and therefore, indirectly, the study of seven transmembrane domain receptors coupled to phospholipase C, receptors having a tyrosine kinase activity, and in general enzymes involved in the variations of the intracellular concentration of IP1.

Inositol-phosphate derivatives and method of detecting inositol-1-phosphate

The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps:

Method of revealing a biological process using a FRET measurement

The invention concerns the use of the fluorescence polarization phenomenon to improve detection of fluorescent signals during a fluorescence resonance energy transfer (FRET). In particular, the invention concerns a method for improving signal/noise ratio in a FRET measurement. The invention also concerns an apparatus for measuring fluorescence following an energy transfer between a donor fluorescent compound and an acceptor fluorescence compound in a measurement medium.

Method for improving the detection of fluorescence signals during a resonance energy transfer

The invention relates to a process for reducing the fluorescence quenching caused by the measuring medium, in a fluorescence assay for an analyte using at least one fluorescent label, characterized in that a fluorescent conjugate comprising an oligonucleotide bonded to a rare-earth metal cryptate is introduced into the measuring medium.

Method for reducing fluorescence quenching in bioassays

The invention relates to a novel homogeneous method of detecting and/or determining the phosphorylating activity of a biological material towards a substrate containing tyrosine and/or serine and/or threonine, and to a kit for carrying out this method.

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Pont Saint Esprit, France

Eric Trinquet