Centre National De La Recherche Scientifique

N.v. Nutricia

Glycom A/s

Institut National De La Sante Et De La Recherche Medicale

Universite Claude Bernard – Lyon1

Centre National De La Recherche Scientifique-cnrs

Ecole Normale Superieure De Lyon

Eric Samain

Bernd Stahl

Johan Garssen

Alma Jildou Nauta

Sophie Drouillard

Gyula Dekany

Elise Champion

Pauline Peltier-Pain

Katrine Bych

Ted Johanson

Margit Pedersen

Manos Papadakis

Peter Becker

Bernard Priem

Mialy Randriantsoa

Emeline Richard

Manuel Rosa-calatrava

Aurélien Traversier

The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.

Oligosaccharide production

The invention concerns nutritional compositions with fucosyllactose for use in stimulation of NK cells. The composition is suitable for infants.

Fucosyllactose as breast milk identical non-digestible oligosaccharide with new functional benefit

Nutritional compositions with fucosyllactose and betagalactooligosaccharides for use in stimulation of the immune system. The composition is suitable for infants.

Mixture of non-digestible oligosaccharides for stimulating the immune system

The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.

The present invention relates to the enzymatic synthesis of oligosaccharides, particularly, sialylated oligosaccharides comprising the carbohydrate moieties of the gangliosides GM3, GD3, and G.

Efficient production of oligosaccharides using metabolically engineered microorganisms

The present invention relates to the enzymatic synthesis of oligosaccharides, particularly, sialylated oligosaccharides comprising the carbohydrate moeities of the gangliosides GM3, GD3, and GT3.

Metabolically engineered microorganisms

Microorganisims used to synthesize oligosaccharides

The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo the oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.

Method for producing oligopolysaccharides

The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.

Method of producing sialylated oligosaccharides

The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.

Growing community of inventors

Gieres, France

Eric Samain