Applied Biosystems, LLC

Applera Corporation

Genapsys, Inc.

The Perkin-elmer Corporation

Life Technologies Corporation

Quantum Biosystems Inc.

Hitachi-high-technologies Corporation

Hitachi, Ltd.

Illumina Cambridge Limited

Pe Corporation (ny)

Eric S Nordman

Mark F Oldham

Richard T Reel

John S Shigeura

Hesaam Esfandyarpour

Kosar Baghbani Parizi

Cheryl Heiner

Frank Lee

Susanne Baumhueter

Janice G Shigeura, Legal Representative

Ryoji Inaba

Steven J Boege

Hongye Sun

Michael W Hunkapiller

Tomoyuki Sakai

Masaya Kojima

Albert L Carrillo

Timothy G Geiser

Paul O Ramstad

John A Bridgham

Stephen B Kent

Shuji Ikeda

Tomoji Kawai

Masateru Taniguchi

Steven Menchen

Linda G Lee

Satoshi Takahashi

Zbigniew T Bryning

Sean Matthew Desmond

Miho Ozawa

Masoud Vakili

Motohiro Yamazaki

Timothy M Woudenberg

Tomohiro Shoji

Kenneth J Livak

Aldrich N K Lau

Paul D Grossman

Yefim M Raysberg

Seiichi Ugai

Charles R Connell

Yasushi Shimizu

Yoshitaka Kodama

Taisaku Seino

Tomonari Morioka

Umberto Ulmanella

Theofilos Kotseroglou

Norman M Whiteley

Louis B Hoff

Dmitry M Sagatelyan

David M Demorest

Philip J Wunderle

Mark P Marriott

Ben F Johnson

Takahito Ohshiro

Howard Gregg King

Shozo Kasai

P Eric Mayrand

John O'Neill

Hisao Kawasaki

Gaurav Goyal

Sam Woo

Willy Wiyatno

Guy R Cathcart

Eric W Lachenmeier

Janice Shigeura, Legal Representative

Kazusuke Mihara

Toshihiko Honkura

Kosar Baghbani Parazi

Aldrich NK Lau

David M Cox

Takehiko Shibasaki

Syouzo Kasai

Ryouji Inaba

Miho Hitachi High-Technologies Corp Ozawa

Yoshitaka Hitachi High-Technologies Corp Kodama

Masaya Hitachi High-Technologies Corp Kojima

Motohiro Hitachi High-Technologies Co Yamazaki

Ryouji Hitachi High-Technologies Corp Inaba

John R O'NEILL

Ryouji c/o Hitachi Ltd Inaba

Yasushi c/o Hitachi Ltd Shimizu

Masaya c/o Hitachi Ltd Kojima

Takehiko c/o Hitachi Ltd Shibasaki

Tomohiro c/o Hitachi Ltd Shoji

Seiichi c/o Hitachi Ltd Ugai

Tomonari c/o Hitachi Ltd Morioka

Syouzo c/o Hitachi Ltd Kasai

Zbigniew Bryning

Sam Lee Woo

The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.

Systems and methods for measurement and sequencing of bio-molecules

There is disclosed a system for electrical charge detection comprising a nanoFET device. Also disclosed is a method of electrical charge detection for single molecule sequencing. The method includes attaching a macromolecule or assemblies thereof to a gate of a nanoFET device and flowing in a solution of charge tags, where a charge tag includes a nucleotide attached to a charge complex. The method also includes incorporating one charge tag into the macromolecule or assemblies thereof and cleaving the charge tags from the macromolecule or assemblies thereof. The method further includes detecting at least one of current and voltage from the nanoFET device.

Systems and methods for electronic detection with nanoFETs

The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Systems and methods for genetic and biological analysis

A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

Scanning system and method for imaging and sequencing

Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Biomolecule sequencing devices, systems and methods

A system for detecting a biomolecule comprises a nano-gap electrode device including a first electrode and a second electrode adjacent to the first electrode. The first electrode can be separated from the second electrode by a nano-gap that is dimensioned to permit the biomolecule to flow through the nano-gap. The nano-gap can have at least a first gap region and a second gap region. The second gap region can be oriented at an angle that is greater than zero degrees with respect to a plane having the first gap region. The system can further include an electrical circuit coupled to the nano-gap electrode device. The electrical circuit can receive electrical signals from the first electrode and the second electrode upon the flow of the biomolecule through the nano-gap.

Nano-gap electrode pair and method of manufacturing same

Systems and methods for electronic detection with nanofets

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Palo Alto, CA, United States of America

Eric S Nordman