Cytek Biosciences, Inc.

Cytek Development Inc

Other

Becton Dickinson and Company Limited

Cytek Corporation

Eric S Chase

Ming Yan

Yung-Chieh Hsieh

David Vrane

Harvey L Schulte

Wenbin Jiang

Howard L North, Jr

Robert A Hoffman

In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

Compact detection module for flow cytometers

A crystal for flow cytometry with dual laser beams is disclosed. The crystal is a birefringent crystal comprising a material composition including a quartz mineral having a face side including a face angle of ninety degrees plus or minus one tenth of a degree; a wedge side that is substantially perpendicular to the face side, wherein the wedge side includes a wedge angle of two degrees plus or minus one tenth of a degree; and a major side that is substantially perpendicular to the face side and the wedge side. The major side includes a thickness of one and one-half millimeter plus or minus one tenth of a millimeter. A polarized light beam entering the birefringent crystal at an incident angle is separated into an ordinary light beam and an extraordinary light beam.

Crystal for flow cytometry with dual laser beams

A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.

Compact multi-color flow cytometer having compact detection module

A system, method, and apparatus are provided for cytometry with dual laser beams. In one example, the method includes directing an incident light beam from a source to enter an optical waveplate; polarizing the incident light beam into a polarized light beam in response to the incident light beam entering through the optical waveplate; directing the polarized light beam to enter a birefringent crystal; separating the polarized light beam into an ordinary light beam and an extraordinary light beam in response to the polarized light beam entering the birefringent crystal; directing the ordinary light beam and the extraordinary light beam to enter a lens; focusing the ordinary light beam and the extraordinary light beam into dual light beams separated by a beam displacement; and coupling the dual light beams to form a sample region having substantially uniform light intensity to analyze moving particles in the particle analyzer.

Flow cytometry with dual laser beams

A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

Compact multi-color flow cytometer

An apparatus and method for examining particles in a flow stream of a flow cytometer, employing incoherent light sources, such as light emitting diodes (LEDs), and detectors. The light emitting diodes operate as the excitation light sources and emit light toward said flow stream, and the detectors detect light, in particular, fluorescent light, emanating from the particles in response to the excitation light striking the particles. A controller controls each of the light emitting diodes to emit their excitation light for a predetermined period during which the excitation light radiates onto particles of interest. The controller evaluates the detected light to ascertain characteristics of the particles, such as particle size, density and granularity. The apparatus and method can further employ one or more coherent and homogenous light emitting devices, such as a laser, as an additional excitation light source. The detectors can detect the LED-excited fluorescence or the laser-excited fluorescence from the particles, and the controller can evaluate both types of detected light to ascertain characteristics of the particles. Furthermore, the controller can control the LEDs to operate in a pulsed manner, which can be synchronized with the detection of the laser-excited fluorescence or light scatter. In addition, a substantially opaque panel having one or more slits can be positioned at the image plane upon which an image of the flow stream is projected, so that the slits will allow only a portion of the image to pass to the detector associated with the panel.

Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer

An apparatus and method are disclosed to wash blood cells in a discrete manner that is compatible with automated sample preparation systems. The test tube containing the cells to be washed is mounted on a rotatable spindle. The spindle includes central passageways for the introduction of wash fluid and air into the test tube, and radial exit passageways at the bottom of the spindle. The test tube is first spun about its vertical axis to centrifuge cells against the inner wall of the test tube. A vacuum is then applied to the exit passageways so cell supernatant is aspirated out through the exit passageways. Wash fluid is then introduced into the test tube, and aspirated out through the exit passageways, thereby washing the cells. Rotational acceleration and deceleration of the test tube then resuspends the cells in wash fluid.

Methods for washing cells

The present invention is a means and method for providing constant output flow from a fluid pump. The invention does this by controlling the radial speed of the pump motor during discreet segments of the motor's 360&deg; angular/radial path through a revolution of the pump. The electric pump motor is controlled throughout the 360&deg; radial path by employing a control means for controlling the speed of actuation of the radial steps of a stepper motor throughout the 360&deg; path of rotation of the stepper motor. Control means for controlling the speed of the discreet steps of the stepper motor comprises at least a memory means, a counting means and an amplification means.

Constant flow fluid pump

Cell wash apparatus

An apparatus and method is disclosed for transferring biological fluids such as blood and serum from closed containers to multiple receiving containers. The disclosed methods and apparatus perform the transfer of an accurate and precise amount of biological liquid without dilution of the biological liquid, and without splattering of the biological liquid on the walls of the receiving container. Furthermore, the method discloses how to obtain equal concentrations of white blood cells in multiple receiving containers characteristic of their concentration in the sealed container.

Growing community of inventors

Walnut Creek, CA, United States of America

Eric S Chase