Synbiotics Corporation

Eric S Bean

Morton A Vodian

Eric D LeMoine

Ronald Sanders

Cheryl Waldman

A saliva enhancement reagent for use in an ELISA wherein a horseradish peroxidase (HRP) enzyme conjugate system is disclosed.

Saliva enhancement reagent for feline leukemia virus elisa

An immunoassay method and apparatus for diagnosing FeLV-induced, persistent viremia is provided comprising combining a feline saliva sample conveniently obtained with a specially designed probe, with anti-p27 antibodies and detecting complex formation as indicative of the viremia. Preferably, the antibodies are bound to the probe for complex formation.

Saliva test for feline leukemia virus

The saliva test for feline leukemia virus (FeLV) employs a probe having an immunochemically sensitive member for collecting saliva from the oral cavity of a cat and employs ELISA reagents for the incubation of the probe and the development of color reactions to indicate the presence or absence of FeLV within the saliva sample collected onto the probe.

The performance of immunoassays for the analysis of serum analytes can be significantly improved by the pretreatment of the sample. Analyte in serum samples is often complexed with serum antibody. Such analyte-antibody complexes can mask the analyte and interfere with analyte specific binding steps of many immunoassays. The serum pretreatment method employs pH dependent chaotropes to dissociate the analyte-antibody complexes in the serum. At low pH, the complexes become dissociated and the antibody becomes denatured. After the dissociation and denaturation of the serum antibody, the serum sample and the chaotrope, contained therein, are then neutralized. Since the serum antibody has been denatured, it does not re-associate with the analyte upon neutralization. After the neutralization step, serum sample can then be analyzed by an immunoassay, without interference from serum antibody. A serum pretreatment kit is essential to the employment of the serum pretreatment method.

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Escondido, CA, United States of America

Eric S Bean