Viridos, Inc.

Synthetic Genomics, Inc.

Exxonmobil Research and Engineering Company

Eric R Moellering

Imad Ajjawi

Saheed Imam

John Verruto

Moena Aqui

Luke Peach

Leah Soriaga

Kathleen Kwok

Nicholas Bauman

Jane C Schneider

Andrew Schultz

Soyan Leung Lieberman

Shaun Bailey

William F Lambert

Roberto Spreafico

Randor R Radakovits

Fedor I Kuzminov

Sarah Potts

Jennifer Nominati

Bo Liu

Erik Holtzapple

Gena Roy

Jessica N Weir

Amanda R Edwards

Amanda Skaggs

Tom Carlson

Ryan Kalb

Wiilliam F Lambert

John H Verruto

Amanda Edwards

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and/or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

Methods for producing a composition containing lipids from recombinant algae having high lipid productivity

The invention involves the provision of recombinant algal mutants that have a genetic modification to a nucleic acid sequence encoding a trehalose biosynthetic enzyme, and/or a genetic modification to a nucleic acid encoding an RNA binding domain And in some embodiments either of these algal mutants can further have a genetic mutation to a nucleic acid sequence encoding an SGI1 polypeptide. Attenuation of one, two, or all three of these genes results in a mutant organism with increased lipid productivity. It was also discovered that one, two, three, or more genetic mutations can be accumulated or 'stacked' in a particular mutant cell or organism to result in further increases in the production of lipid products. The lipid products of these mutants are useful as biofuels or for other specialty chemical products.

Recombinant algae having high lipid productivity

The invention provides recombinant algal organisms that have a genetic modification to a gene or nucleic acid sequence encoding an RNA binding domain. In some embodiments the genetic modification can be a functional deletion or attenuation of the gene. The genetic modification results in a mutant organism with increased lipid productivity and/or higher biomass productivity. The lipid products of these mutants can be utilized as biofuels or to manufacture other specialty products. The recombinant mutants can also, optionally, have a genetic modification to a gene encoding an SGI1 polypeptide. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines and methods of generating mutations in the genome of an organism using such cell lines.

RNA-guided endonuclease expressing algal strain for high efficiency in vivo genome editing

The invention involves the provision of recombinant algal mutants that have a genetic modification to a nucleic acid sequence encoding a trehalose biosynthetic enzyme, and/or a genetic modification to a nucleic acid encoding an RNA binding domain. And in some embodiments either of these algal mutants can further have a genetic mutation to a nucleic acid sequence encoding an SGI1 polypeptide. Attenuation of one, two, or all three of these genes results in a mutant organism with increased lipid productivity. It was also discovered that one, two, three, or more genetic mutations can be accumulated or 'stacked' in a particular mutant cell or organism to result in further increases in the production of lipid products. The lipid products of these mutants are useful as biofuels or for other specialty chemical products.

Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

High productivity algal mutants having reduced photosynthetic antenna

The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines.

Compositions and methods for high efficiency in vivo genome editing

The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while biomass at levels that are within approximately 50% of wild type biomass productivities under nitrogen replete conditions. Particular mutants produce at least twice as much FAME lipid as wild type while producing at least 75% of the biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

Microorganisms having increased lipid productivity

The present invention provides mutant microorganisms having attenuated expression of a gene encoding a polypeptide that includes a TPR domain, wherein the mutant microorganisms have higher lipid productivity and/or exhibit increased partitioning of carbon to lipid as compared to wild type microorganisms from which they are derived. Also provided are methods of producing lipids using the mutant microorganisms, guide RNAs, and nucleic acid constructs used for producing mutant microorganisms.

Algal lipid productivity via genetic modification of a TPR domain containing protein

The present invention provides a mutant algal microorganism that has a mutation that causes attenuated expression of TrifuncB and/or TrifuncA and as a result produces more lipids than a control algal microorganism. The mutant algal microorganism can further include a mutation in a gene encoding a peroxisomal beta-oxidation pathway protein, such as an ACO1 or PXA1 gene, or a glyoxylate pathway protein, such as an ICL1 gene, that results in attenuated expression and further increased lipid production. Furthermore, provided herein are methods of producing lipids using the mutant algal microorganisms and methods of making the mutant microorganisms.

Recombinant algal microorganisms having increased lipid production, and methods of making and using the same

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while producing biomass at levels that are at least 45% of wild type biomass productivity under nitrogen replete conditions. Particular mutants produce at least 50% as much FAME lipid as wild type while producing at least the amount of biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.

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San Diego, CA, United States of America

Eric R Moellering