Leidos, Inc.

Eric Matthew Amendt

Richard Holmes Griffey

William Leslie Hicks

John Dresios

Ryan Shyffer

Henri M Sasmor

Challise J Sullivan

Erik D Pendleton

A portable fluidic platform for rapid and flexible end-to-end production of recombinant protein biologics includes a bioreactor system hosting stable and robust cell-free translation systems that is fluidically integrated with modular protein separation functionalities (e.g., size exclusion, ion exchange or affinity chromatography systems) for purification of the cell-free expressed product and which are configurable for process-specific isolation of different proteins, as well as for formulation. The bioreactor utilizes lysates from engineered eukaryotic (e.g., yeast) or prokaryotic (e.g., bacterial) strains that contain factors for protein folding and posttranslational modifications. Combination of various purification modules on the same fluidic platform allows flexibility of re-routing for purification of different proteins depending on specific target requirements. Protein synthesis and purification modules are integrated into self-contained disposable fluidic cartridge that eliminates cross-contamination between runs. The platform allows for flexible production of protein biologics within 24 hours (from DNA to purified product).

Portable fluidic platform for rapid cell-free production of protein biologics

A Geiger-Mueller charge particle rate measurement system includes a clock management unit in combination with multiple oscillators and rate feedback controller to allow for reactive switching between the different oscillator frequencies to optimize system use. Controlling the clock management unit to send the appropriate frequency (clock signal) to the timers in response to measured rate date from the rate feedback controller facilitates operation at different clock speeds, which helps reduce power consumption when operated at lower speeds.

Switching frequency extended range Geiger detection system and method

Switching frequency extended range geiger detection system and method

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San Diego, CA, United States of America

Eric Matthew Amendt