Centre National De La Recherche Scientifique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Total Raffinage Chimie

Fermentalg

Université Joseph Fourier

Eric Marechal

Jacques Joyard

Cedric Allier

Jean-Christophe Cintrat

Cédric Allier

Pierre Calleja

Jérôme Boutet

Lina Juana Dolch

Nadia Saidani

Cyrille Botte

Giovanni Finazzi

Marina Leterrier

Fabrice Rebeille

Juliette Jouhet

Laurent Fourage

Caroline Barette

Jerome Boutet

Bernard Rousseau

Henri Joseph Vial

Stéphane Miras

Frédéric Laeuffer

Roland Douce

Henri Vial

Leonardo Magneschi

Jean-François Dubremetz

Maryse Block

Marine Aimée Peuchmaur

Roman Lopez

Hélène Hardre

Melissa Conte

Michael Deligny

Anne-Laure Bonneau

Corinne Mercier

Mariette Bedhomme

Lina Dolch

Séverine Collin

Camille Rak

Denis Falconet

Dimitris Petroutsos

Coline Mei

Lina-Juana Dolch

Marie-France Cesbron-Delauw

The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of(NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.

Chloroplast targeting peptide sequence derived from nannochloropsis phosphoribulokinase and methods for use

The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.

Use of nitric oxide or nitric oxide donor for inducing the production of triacylglycerols in microalgae

The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.

Inhibitors of sterol metabolism for their use to accumulate triglycerides in microalgae, and methods thereof

The present technology relates to a microalga that is genetically modified by inhibiting the activity of the enzyme &#x394;0-elongase (&#x394;0-ELO), which uses palmitic acid, a saturated fatty acid, as substrate and is associated with the production of monogalactosyldiacylglycerol (MGDG). The present technology also relates to a process for culturing the microalga for increased production of triacylglycerols (TAGs) and recovery of the TAGs.

Alga modified for increased tag production

The application generally relates to bioproduction of molecules of interest in micro-organisms, more particularly in microalgae. In particular, the application relates to methods for increasing triacylglycerol production in micro-organisms, in particular in microalgae, using recombinant micro-organisms which have been genetically engineered to produce or overproduce nitric oxide (NO) and uses thereof.

Increased triacylglycerol production in microalgae

A method and a device for determining a concentration of lipids in a microorganism such as a micro-alga by illuminating a sample containing microorganisms and acquiring a total diffraction pattern of the sample is acquired, the total diffraction pattern including a plurality of unit diffraction pattern each associated with a microorganism.

Method for determining a concentration of lipids in a microorganism

The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.

Modified algae strain and method of triacylglycerol accumulation using said strain

The invention relates to the use of compounds having a monogalactosyldiacylglycerol (MGDG) synthase inhibitory activity as herbicide or algaecide, and to herbicide and algaecide compositions containing at least one of these compounds.

Compounds having a monogalactosyldiacylglycerol synthase inhibitory activity as herbicide or algaecide, herbicide and algaecide compositions

Novel polyspirane compounds used in the treatment of diseases involving parasites that belong to the phylum of apicomplexae, and a method for preparing the same.

Polyspirane compounds, application thereof in the treatment of malaria or toxoplasmosis and method for preparing same

The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.

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The invention concerns a method for screening and selecting parasiticides (apicomplex phyllum parasites) and/or herbicides and the uses thereof. Said method consists in incubating a substance to be tested with a MGDG synthase and measuring the specific enzymatic activity, after said incubation. The invention also concerns the use of MGDG synthase or a plant isolated plastid membrane for selecting or screening the products inhibiting the activity of the MGDG synthase, capable of being used as active principles against apicomplex parasites and/or of being used as herbicides.

Screening method involving MGDG synthase

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Grenoble, France

Eric Marechal