Pierce Biotechnology, Inc.

Life Technologies Holdings Pte Limited

Eric Leigh Hommema

Suk Hong

Nikki L Jarrett

Brian Webb

John Charles Rogers

Ryan Daniel Bomgarden

Kelli D Feather-Henigan

Rachael Mae Berry

Jason Aravich

Surbhi Desai

Boguslawa Dworecki

Kok Siong Teo

Christopher Laurence Etienne

Gregory John Kilmer

Benyong Shi

Peter Alan Bell

Yanpeng Cao

Hongfang Wang

Penny JoAnn Jensen

Derek Karl Baerenwald

John David Dwenger

Stefan Reiley Freeman

Priya Rangaraj

Stephen D Shiflett

A method for creating a projected image including capturing an image of an item or data relating to an image of an item with an image capture device having a plurality of light sensing units, at a first exposure time. The method further includes receiving as an input or determining a second exposure time, and creating a projected image of the item at the second exposure time based upon the captured image or the captured data relating to the image.

Method and system for projecting image with differing exposure times

An electroblotting system including a cassette configured to receive a membrane and a material impregnated with at least one of proteins or nucleic acids. The cassette is connectable to a base such that a current is passable through the cassette to cause at least some of the proteins or nucleic acids to be transferred from the impregnated material to the membrane. The cassette has a first portion, a second portion configured to be releasably coupled to the first portion, and a coupling mechanism configured to releasably couple the first portion to the second portion. The cassette further includes an actuator operatively coupled to the coupling mechanism such that manual depression of the actuator causes the coupling mechanism to release the first portion relative to the second portion.

Electroblotting device

Systems and methods generate a projected image at an optimal exposure time. Images are captured different exposure times. Pixels that satisfy an intensity threshold percentage for each image are selected. The intensity values of the selected pixels are then evaluated to determine whether the selected pixels are distributed above a lower intensity threshold and below an upper intensity threshold. The linear relationship is projected to determine an optimal exposure time that has an optimal exposure time duration that exceeds each exposure time duration associated with each of the captured images when the linear relationship exists between each of the captured images. A projected image associated with the optimal exposure time is generated from one or more of the captured images.

Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.

Methods and reagents for biomolecule labeling, enrichment and gentle elution

A method and system to enhance analysis of electrophoretic bands by overlaying only the pixels of interest. The overlaid pixels are superimposed as a layer above, i.e., in the foreground of, the overlaid image, i.e., in the background. A user employs the superimposed pixels for molecular weight determination and is still able to generate densitometry analysis of the remaining pixels in the overlaid image.

Analysis of electrophoretic bands in a substrate

Methods for producing an isotope-labeled mammalian, including a human, biomolecule, such as polypeptides and proteins, in a cell-free protein synthesis system. A biomolecule standard is produced having at least one isotope different in abundance than that of the naturally occurring isotopes in the biomolecule. Methods for quantifying biomolecules standards expressed using mammalian cell-free extracts are disclosed. Methods for producing such standards, kits, systems and reagents, relating to the use of isotope-labeled biomolecule as quantification standards in mass spectrometric and nuclear magnetic resonance analysis.

Methods for isotopically labeling biomolecules using mammalian cell-free extracts

Growing community of inventors

Roscoe, IL, United States of America

Eric Leigh Hommema